ArticleBMC microbiology2008
Development of a luciferase-based reporter system to monitor Bifidobacterium breve UCC2003 persistence in mice.
Article in BMC microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Harnessing gut microbes for glycan detection and quantification.Nature communications · 2023Article
- Spatial and Temporal Persistence of FluorescentFrontiers in microbiology · 2022Article
- Recombinant lactic acid bacteria as promising vectors for mucosal vaccination.Exploration (Beijing, China) · 2021Review
- Construction of a reporter system for bifidobacteria using chloramphenicol acetyltransferase and its application for evaluation of promoters and terminators.Bioscience of microbiota, food and health · 2021Article
- A Resource for Cloning and Expression Vectors Designed for Bifidobacteria: Overview of Available Tools and Biotechnological Applications.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Bifidobacterium breve UCC2003 Induces a Distinct Global Transcriptomic Program in Neonatal Murine Intestinal Epithelial Cells.iScience · 2020Article
- In vivo bioluminescence imaging of the spatial and temporal colonization of lactobacillus plantarum 423 and enterococcus mundtii ST4SA in the intestinal tract of mice.BMC microbiology · 2018Article
- Construction of a Bioluminescent Labelling Plasmid Vector for Bifidobacteria.Korean journal for food science of animal resources · 2018Article
- Fluorescent Lactic Acid Bacteria and Bifidobacteria as Vehicles of DNA Microbial Biosensors.International journal of molecular sciences · 2017Article
- Monitoring of BioluminescentKorean journal for food science of animal resources · 2017Article
- Gene Replacement and Fluorescent Labeling to Study the Functional Role of Exopolysaccharides inFrontiers in microbiology · 2017Article
- Fluorescent reporter systems for tracking probiotic lactic acid bacteria and bifidobacteria.World journal of microbiology & biotechnology · 2016Review
- Use of the mCherry Fluorescent Protein To Study Intestinal Colonization by Enterococcus mundtii ST4SA and Lactobacillus plantarum 423 in Mice.Applied and environmental microbiology · 2015Article
- Tn5/7-lux: a versatile tool for the identification and capture of promoters in gram-negative bacteria.BMC microbiology · 2015Article
- Under the microscope: From pathogens to probiotics and back.Bioengineered · 2015Review
- Reporter systems for in vivo tracking of lactic acid bacteria in animal model studies.Gut microbes · 2015Review
- Expression of fluorescent proteins in bifidobacteria for analysis of host-microbe interactions.Applied and environmental microbiology · 2014Article
- Bioluminescence imaging study of spatial and temporal persistence of Lactobacillus plantarum and Lactococcus lactis in living mice.Applied and environmental microbiology · 2013Article
- Accessing the inaccessible: molecular tools for bifidobacteria.Applied and environmental microbiology · 2012Review
- High resolution in vivo bioluminescent imaging for the study of bacterial tumour targeting.PloS one · 2012Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundProbiotics such as bifidobacteria have been shown to maintain a healthy intestinal microbial balance and help protect against infections. However, despite these benefits, bifidobacteria still remain poorly understood at the biochemical, physiological and especially the genetic level. Herein we describe, for the first time, the development of a non-invasive luciferase-based reporter system for real-time tracking of Bifidobacterium species in vivo.
resultsThe reporter vector pLuxMC1 is based on the recently described theta-type plasmid pBC1 from B. catenatulatum 1 and the luxABCDE operon from pPL2lux 2. Derivatives of pLuxMC1, harbouring a bifidobacterial promoter (pLuxMC2) as well as a synthetically derived promoter (pLuxMC3) 3 placed upstream of luxABCDE, were constructed and found to stably replicate in B. breve UCC2003. The subsequent analysis of these strains allowed us to assess the functionality of pLuxMC1 both in vitro and in vivo.
conclusionOur results demonstrate the potential of pLuxMC1 as a real-time, non-invasive reporter system for Bifidobacterium. It has also allowed us, for the first time, to track the colonisation potential and persistence of this probiotic species in real time. An interesting and significant outcome of the study is the identification of the caecum as a niche environment for B. breve UCC2003 within the mouse gastrointestinal tract (GI) tract.
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