ArticleParasitology research2009
Reference genes for quantitative analysis on Clonorchis sinensis gene expression by real-time PCR.
Article in Parasitology research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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45 citing papers in PubMed, 66 citations in OpenAlex.
- Selection and Validation of Reference Genes inPlants (Basel, Switzerland) · 2024Article
- Clonorchis sinensis legumain promotes migration and invasion of cholangiocarcinoma cells via regulating tumor-related molecules.Parasites & vectors · 2023Article
- Selection of the reference genes for quantitative gene expression by RT-qPCR in the desert plant Stipagrostis pennata.Scientific reports · 2021Article
- Article
- Sodium-bile acid co-transporter is crucial for survival of a carcinogenic liver fluke Clonorchis sinensis in the bile.PLoS neglected tropical diseases · 2020Article
- Transcriptomic Features of Echinococcus granulosus Protoscolex during the Encystation Process.The Korean journal of parasitology · 2020Article
- Clonorchis sinensis secretory protein CsAg17 vaccine induces immune protection.Parasites & vectors · 2020Article
- Amino acids serve as an important energy source for adult flukes of Clonorchis sinensis.PLoS neglected tropical diseases · 2020Article
- Reference gene analysis and its use for kinase expression profiling in Fasciola hepatica.Scientific reports · 2019Article
- Identification of reliable reference genes for quantitative real-time PCR normalization in pitaya.Plant methods · 2019Article
- Characterization of a novel organic solute transporter homologue from Clonorchis sinensis.PLoS neglected tropical diseases · 2018Article
- Multidrug resistance-associated protein 4 is a bile transporter of Clonorchis sinensis simulated by in silico docking.Parasites & vectors · 2017Article
- Molecular and structural characteristics of multidrug resistance-associated protein 7 in Chinese liver fluke Clonorchis sinensis.Parasitology research · 2017Article
- Identification of Optimal Reference Genes for Normalization of qPCR Analysis during Pepper Fruit Development.Frontiers in plant science · 2017Article
- Genome-Wide Identification and Evaluation of Reference Genes for Quantitative RT-PCR Analysis during Tomato Fruit Development.Frontiers in plant science · 2017Article
- Identification of Reference Genes for Quantitative Gene Expression Studies in a Non-Model Tree Pistachio (Pistacia vera L.).PloS one · 2016Article
- Clonorchis sinensis acetoacetyl-CoA thiolase: identification and characterization of its potential role in surviving in the bile duct.Parasites & vectors · 2015Article
- Identification and molecular characterization of Parkin in Clonorchis sinensis.The Korean journal of parasitology · 2015Article
- Selection of suitable reference genes for qPCR normalization under abiotic stresses and hormone stimuli in carrot leaves.PloS one · 2015Article
- Expression profiles of glyceraldehyde-3-phosphate dehydrogenase from Clonorchis sinensis: a glycolytic enzyme with plasminogen binding capacity.Parasitology research · 2014Article
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The accuracies of relative gene expressions as determined by quantitative real-time polymerase chain reaction are largely dependent on the variabilities of the reference genes used. Validation of the stabilities of reference genes under experimental conditions is an essential initial step for comparative studies on the expression levels of target genes in experimental groups. Using three total RNA samples extracted independently from Clonorchis sinensis metacercariae and adults, we determined the gene expression stabilities of eight reference gene candidates and the relative transcript levels of three target genes using the geNorm program. The reference genes found to be stably expressed in metacercariae and adults were phosphoglycerate kinase, beta-actin, and calcyphosine; reference genes found to be stably expressed under gamma-irradiated and non-irradiated conditions were succinate dehydrogenase, small nuclear ribonucleoprotein, and beta-actin; and those stably expressed regardless of bile treatment were small nuclear ribonucleoprotein, phosphoglycerate kinase, and succinate dehydrogenase. According to our data, the expression levels of target genes are dependent on normalization factors, such as the C (T) values of single reference genes and the geometric mean of the C (T) values of three reference genes. When comparing C. sinensis gene expressions, we propose to employ the geometric mean of the C (T) values of more than three reference genes validated in the same experimental setting.
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