ArticleJournal of virology2008
Murine coronaviruses encoding nsp2 at different genomic loci have altered replication, protein expression, and localization.
Article in Journal of virology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Novel Mutations in the Non-Structure Protein 2 of SARS-CoV-2.Mediterranean journal of hematology and infectious diseases · 2023Article
- Host and Viral Zinc-Finger Proteins in COVID-19.International journal of molecular sciences · 2022Review
- Genome composition and genetic characterization of SARS-CoV-2.Saudi journal of biological sciences · 2021Review
- Functional and druggability analysis of the SARS-CoV-2 proteome.European journal of pharmacology · 2021Article
- Understanding COVID-19 in Wuhan From the Perspective of Cold-Dampness: Clinical Evidences and Mechanisms.Frontiers in medicine · 2021Review
- COVID-19: Emergence of Infectious Diseases, Nanotechnology Aspects, Challenges, and Future Perspectives.ChemistrySelect · 2020Review
- Emerging coronaviruses: Genome structure, replication, and pathogenesis.Journal of medical virology · 2020Review
- Severe Acute Respiratory Syndrome Coronavirus 2: From Gene Structure to Pathogenic Mechanisms and Potential Therapy.Frontiers in microbiology · 2020Review
- An updated analysis of variations in SARS-CoV-2 genome.Turkish journal of biology = Turk biyoloji dergisi · 2020Article
- Article
- Jumping species-a mechanism for coronavirus persistence and survival.Current opinion in virology · 2017Review
- Coronavirus replicase-reporter fusions provide quantitative analysis of replication and replication complex formation.Journal of virology · 2014Article
- Review
- Purification, crystallization and preliminary X-ray analysis of nonstructural protein 2 (nsp2) from avian infectious bronchitis virus.Acta crystallographica. Section F, Structural biology and crystallization communications · 2012Article
- A new cistron in the murine hepatitis virus replicase gene.Journal of virology · 2010Article
- Dynamics of coronavirus replication-transcription complexes.Journal of virology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Partial or complete deletion of several coronavirus nonstructural proteins (nsps), including open reading frame 1a (ORF1a)-encoded nsp2, results in viable mutant proteins with specific replication defects. It is not known whether expression of nsps from alternate locations in the genome can complement replication defects. In this report, we show that the murine hepatitis virus nsp2 sequence was tolerated in ORF1b with an in-frame insertion between nsp13 and nsp14 and in place of ORF4. Alternate encoding or duplication of the nsp2 gene sequence resulted in differences in nsp2 expression, processing, and localization, was neutral or detrimental to replication, and did not complement an ORF1a Deltansp2 replication defect. The results suggest that wild-type genomic organization and expression of nsps are required for optimal replication.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.