ArticleAmerican journal of physiology. Cell physiology2008
Human neutrophil surface protrusion under a point load: location independence and viscoelasticity.
Article in American journal of physiology. Cell physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Acoustic radiation force on a long cylinder, and potential sound transduction by tomato trichomes.Biophysical journal · 2022Article
- Viscoelasticity, Like Forces, Plays a Role in Mechanotransduction.Frontiers in cell and developmental biology · 2022Review
- The Mechanics and Thermodynamics of Tubule Formation in Biological Membranes.The Journal of membrane biology · 2021Review
- Biomechanics of Neutrophil Tethers.Life (Basel, Switzerland) · 2021Review
- Squeezing through the microcirculation: survival adaptations of circulating tumour cells to seed metastasis.British journal of cancer · 2021Review
- Microfluidics-based side view flow chamber reveals tether-to-sling transition in rolling neutrophils.Scientific reports · 2016Article
- Flexural Rigidity and Shear Stiffness of Flagella Estimated from Induced Bends and Counterbends.Biophysical journal · 2016Article
- Endothelial Surface Protrusion by a Point Force.Biophysical journal · 2016Article
- Neutrophil rolling at high shear: flattening, catch bond behavior, tethers and slings.Molecular immunology · 2013Review
- Effects of plasma membrane cholesterol level and cytoskeleton F-actin on cell protrusion mechanics.PloS one · 2013Article
- Article
- Cell protrusions and tethers: a unified approach.Biophysical journal · 2011Article
- Biomechanics of leukocyte rolling.Biorheology · 2011Review
- A semianalytical model to study the effect of cortical tension on cell rolling.Biophysical journal · 2010Article
- Unfolding the A2 domain of von Willebrand factor with the optical trap.Biophysical journal · 2010Article
- Validation, In-Depth Analysis, and Modification of the Micropipette Aspiration Technique.Cellular and molecular bioengineering · 2009Article
- Membrane tubulovesicular extensions (cytonemes): secretory and adhesive cellular organelles.Cell adhesion & migrationReview
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Mechanical properties of neutrophils have been recognized as key contributors to stabilizing neutrophil rolling on the endothelium during the inflammatory response. In particular, accumulating evidence suggests that surface protrusion and tether extraction from neutrophils facilitate stable rolling by relieving the disruptive forces on adhesive bonds. Using a customized optical trap setup, we applied piconewton-level pulling forces on targeted receptors that were located either on the microvillus tip (CD162) or intermicrovillus surface of neutrophils (CD18 and CD44). Under a constant force-loading rate, there always occurred an initial tent-like surface protrusion that was terminated either by rupture of the adhesion or by a "yield" or "crossover" to tether extraction. The corresponding protrusional stiffness of neutrophils was found to be between 0.06 and 0.11 pN/nm, depending on the force-loading rate and the cytoskeletal integrity, but not on the force location, the medium osmolality, nor the temperature increase from 22 degrees C to 37 degrees C. More importantly, we found that neutrophil surface protrusion was accompanied by force relaxation and hysteresis. In addition, the crossover force did not change much in the range of force-loading rates studied, and the protrusional stiffness of lymphocytes was similar to that of neutrophils. These results show that neutrophil surface protrusion is essentially viscoelastic, with a protrusional stiffness that stems primarily from the actin cortex, and the crossover force is independent of the receptor-cytoskeleton interaction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.