ArticleAmerican journal of physiology. Cell physiology2008
Regulation of cell proliferation by intermediate-conductance Ca2+-activated potassium and volume-sensitive chloride channels in mouse mesenchymal stem cells.
Article in American journal of physiology. Cell physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 85 citations in OpenAlex.
- Hyperpolarization by Optogenetic Activation of NpHR Channels Promotes Osteogenic Differentiation of Human Dental Follicle Stem Cells.Membranes · 2026Article
- Intermediate Conductance Calcium-Activated Potassium Channel Activation in Stem Cell Antigen-1 Positive Cells Contributed to Vascular Restenosis.Journal of the American Heart Association · 2026Article
- CaInternational journal of molecular sciences · 2023Review
- TWIK-related acid-sensitive KiScience · 2022Article
- Downregulation of the Proton-Activated Cl- Channel TMEM206 Inhibits Malignant Properties of Human Osteosarcoma Cells.Oxidative medicine and cellular longevity · 2021Article
- How TRPC Channels Modulate Hippocampal Function.International journal of molecular sciences · 2020Review
- A critical role of the KJournal of cellular and molecular medicine · 2020Article
- KJournal of the American Heart Association · 2019Article
- Article
- Calcium-dependent potassium channels control proliferation of cardiac progenitor cells and bone marrow-derived mesenchymal stem cells.The Journal of physiology · 2018Article
- NPPB modulates apoptosis, proliferation, migration and extracellular matrix synthesis of conjunctival fibroblasts by inhibiting PI3K/AKT signaling.International journal of molecular medicine · 2018Article
- Article
- Ion channel signaling influences cellular proliferation and phagocyte activity during axolotl tail regeneration.Mechanisms of development · 2017Article
- Bradykinin regulates cell growth and migration in cultured human cardiac c-Kit+ progenitor cells.Oncotarget · 2017Article
- TRPC1/TRPC3 channels mediate lysophosphatidylcholine-induced apoptosis in cultured human coronary artery smooth muscles cells.Oncotarget · 2016Article
- ClC-3 Chloride Channel Proteins Regulate the Cell Cycle by Up-regulating cyclin D1-CDK4/6 through Suppressing p21/p27 Expression in Nasopharyngeal Carcinoma Cells.Scientific reports · 2016Article
- The role of KCa3.1 channels in cardiac fibrosis induced by pressure overload in rats.Pflugers Archiv : European journal of physiology · 2015Article
- X-ray irradiation activates K+ channels via H2O2 signaling.Scientific reports · 2015Article
- Complementary roles of KCa3.1 channels and β1-integrin during alveolar epithelial repair.Respiratory research · 2015Article
- KCa3.1/IK1 Channel Regulation by cGMP-Dependent Protein Kinase (PKG) via Reactive Oxygen Species and CaMKII in Microglia: An Immune Modulating Feedback System?Frontiers in immunology · 2015Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Bone marrow mesenchymal stem cells (MSCs) are a promising cell source for regenerative medicine; however, their cellular physiology is not fully understood. The present study aimed at exploring the potential roles of the two dominant functional ion channels, intermediate-conductance Ca(2+)-activated potassium (IK(Ca)) and volume-sensitive chloride (I(Cl.vol)) channels, in regulating proliferation of mouse MSCs. We found that inhibition of IK(Ca) with clotrimazole and I(Cl.vol) with 5-nitro-1-(3-phenylpropylamino) benzoic acid (NPPB) reduced cell proliferation in a concentration-dependent manner. Knockdown of KCa3.1 or Clcn3 with specific short interference (si)RNAs significantly reduced IK(Ca) or I(Cl.vol) density and channel protein and produced a remarkable suppression of cell proliferation (by 24.4 +/- 9.6% and 29.5 +/- 7.2%, respectively, P < 0.05 vs. controls). Flow cytometry analysis showed that mouse MSCs retained at G(0)/G(1) phase (control: 51.65 +/- 3.43%) by inhibiting IK(Ca) or I(Cl.vol) using clotrimazole (2 microM: 64.45 +/- 2.20%, P < 0.05) or NPPB (200 microM: 82.89 +/- 2.49%, P < 0.05) or the specific siRNAs, meanwhile distribution of cells in S phase was decreased. Western blot analysis revealed a reduced expression of the cell cycle regulatory proteins cyclin D1 and cyclin E. Collectively, our results have demonstrated that IK(Ca) and I(Cl.vol) channels regulate cell cycle progression and proliferation of mouse MSCs by modulating cyclin D1 and cyclin E expression.
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