ArticleAmerican journal of physiology. Cell physiology2008
Anisotropic diffusion of fluorescently labeled ATP in rat cardiomyocytes determined by raster image correlation spectroscopy.
Article in American journal of physiology. Cell physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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49 citing papers in PubMed, 72 citations in OpenAlex.
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- Ontogeny of cardiomyocytes: ultrastructure optimization to meet the demand for tight communication in excitation-contraction coupling and energy transfer.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Calcium Signaling in the Photodamaged Skin: In Vivo Experiments and Mathematical Modeling.Function (Oxford, England) · 2022Article
- Energy metabolism design of the striated muscle cell.Physiological reviews · 2021Review
- Co-localization and confinement of ecto-nucleotidases modulate extracellular adenosine nucleotide distributions.PLoS computational biology · 2020Article
- Respiration of permeabilized cardiomyocytes from mice: no sex differences, but substrate-dependent changes in the apparent ADP-affinity.Scientific reports · 2019Article
- Consequences of Heterogeneous Crowding on an Enzymatic Reaction: A Residence Time Monte Carlo Approach.ACS omega · 2019Article
- Direct Quantification of Solute Diffusivity in Agarose and Articular Cartilage Using Correlation Spectroscopy.Annals of biomedical engineering · 2017Article
- Energy Requirements of Odor Transduction in the Chemosensory Cilia of Olfactory Sensory Neurons Rely on Oxidative Phosphorylation and Glycolytic Processing of Extracellular Glucose.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Power Grid Protection of the Muscle Mitochondrial Reticulum.Cell reports · 2017Article
- Quantifying the Influence of the Crowded Cytoplasm on Small Molecule Diffusion.The journal of physical chemistry. B · 2016Article
- Enzyme localization, crowding, and buffers collectively modulate diffusion-influenced signal transduction: Insights from continuum diffusion modeling.The Journal of chemical physics · 2015Article
- Universal Approach to FRAP Analysis of Arbitrary Bleaching Patterns.Scientific reports · 2015Article
- Photothermal raster image correlation spectroscopy of gold nanoparticles in solution and on live cells.Royal Society open science · 2015Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
A series of experimental data points to the existence of profound diffusion restrictions of ADP/ATP in rat cardiomyocytes. This assumption is required to explain the measurements of kinetics of respiration, sarcoplasmic reticulum loading with calcium, and kinetics of ATP-sensitive potassium channels. To be able to analyze and estimate the role of intracellular diffusion restrictions on bioenergetics, the intracellular diffusion coefficients of metabolites have to be determined. The aim of this work was to develop a practical method for determining diffusion coefficients in anisotropic medium and to estimate the overall diffusion coefficients of fluorescently labeled ATP in rat cardiomyocytes. For that, we have extended raster image correlation spectroscopy (RICS) protocols to be able to discriminate the anisotropy in the diffusion coefficient tensor. Using this extended protocol, we estimated diffusion coefficients of ATP labeled with the fluorescent conjugate Alexa Fluor 647 (Alexa-ATP). In the analysis, we assumed that the diffusion tensor can be described by two values: diffusion coefficient along the myofibril and that across it. The average diffusion coefficients found for Alexa-ATP were as follows: 83 +/- 14 microm(2)/s in the longitudinal and 52 +/- 16 microm(2)/s in the transverse directions (n = 8, mean +/- SD). Those values are approximately 2 (longitudinal) and approximately 3.5 (transverse) times smaller than the diffusion coefficient value estimated for the surrounding solution. Such uneven reduction of average diffusion coefficient leads to anisotropic diffusion in rat cardiomyocytes. Although the source for such anisotropy is uncertain, we speculate that it may be induced by the ordered pattern of intracellular structures in rat cardiomyocytes.
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