ArticleCardiovascular research2009
Interaction between transcription factors Iroquois proteins 4 and 5 controls cardiac potassium channel Kv4.2 gene transcription.
Article in Cardiovascular research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 42 citations in OpenAlex.
- Article
- TheiScience · 2025Article
- IRX2 regulates angiotensin II-induced cardiac fibrosis by transcriptionally activating EGR1 in male mice.Nature communications · 2023Article
- Article
- Understanding the role of Iroquois homeobox transcription factor 5 (IRX5) in cardiac function: getting to the (human) heart of the matter.Cardiovascular research · 2021Article
- IRX3 and IRX5 collaborate during ovary development and follicle formation to establish responsive granulosa cells in the adult mouse†.Biology of reproduction · 2020Article
- Article
- Id2 Determines Intestinal Identity through Repression of the Foregut Transcription Factor Irx5.Molecular and cellular biology · 2018Article
- Iroquois Homeodomain transcription factors in ventricular conduction system and arrhythmia.International journal of medical sciences · 2018Review
- Loss of Iroquois homeobox transcription factors 3 and 5 in osteoblasts disrupts cranial mineralization.Bone reports · 2016Article
- Iroquois homeobox transcription factor (Irx5) promotes G1/S-phase transition in vascular smooth muscle cells by CDK2-dependent activation.American journal of physiology. Cell physiology · 2016Article
- Feedback mechanisms for cardiac-specific microRNAs and cAMP signaling in electrical remodeling.Circulation. Arrhythmia and electrophysiology · 2015Article
- Nkx genes are essential for maintenance of ventricular identity.Development (Cambridge, England) · 2013Article
- Hyperoxia-induced hypertrophy and ion channel remodeling in left ventricle.American journal of physiology. Heart and circulatory physiology · 2013Article
- Distinctive profile of IsomiR expression and novel microRNAs in rat heart left ventricle.PloS one · 2013Article
- MicroRNA-301a mediated regulation of Kv4.2 in diabetes: identification of key modulators.PloS one · 2013Article
- Cooperative and antagonistic roles for Irx3 and Irx5 in cardiac morphogenesis and postnatal physiology.Development (Cambridge, England) · 2012Article
- Initial deployment of the cardiogenic gene regulatory network in the basal chordate, Ciona intestinalis.Developmental biology · 2012Article
- High-purity enrichment of functional cardiovascular cells from human iPS cells.Cardiovascular research · 2012Article
- Gene regulatory networks in cardiac conduction system development.Circulation research · 2012Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
aimsThe homeobox transcription factor, Iroquois protein 5 (Irx5), plays an essential role in the generation of region-selective expression of Kv4.2 gene across the left ventricular wall of rodent hearts. Here, we analyse molecular mechanisms underlying the Irx5-induced regulation of the rat Kv4.2 promoter. METHODS AND
resultsThe mRNA levels for Irx members in various heart regions were assessed by RT-PCR. A luciferase reporter gene with the rat Kv4.2 promoter was used to test the effects of Irx members on channel promoter activity. Irx3 and Irx5 mRNAs were differentially distributed across the left ventricular wall, whereas Irx4 message was equally abundant in various ventricular regions. Irx5, but not Irx3 or Irx4, increased Kv4.2 promoter activity in 10T1/2 fibroblasts, whereas the transcription factor decreased promoter activity in neonatal ventricular myocytes. These effects were mediated by the C-terminal portion of Irx5. Irx4 appeared to inhibit the Irx5-induced increase in channel promoter activity in 10T1/2 cells. The N-terminal region of Irx4 was necessary and sufficient for this inhibition. Furthermore, when endogenous Irx4 expression was suppressed with siRNA, Irx5 increased channel promoter activity in neonatal myocytes.
conclusionThese results indicate that Irx5 possesses the ability to activate the Kv4.2 promoter. The abundant Irx4 expression throughout the rat ventricle may play a role in the inverse relationship between Irx5 and Kv4.2 levels across the left ventricular wall.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.