ArticleProceedings of the National Academy of Sciences of the United States of America2008
A channel connecting the mother cell and forespore during bacterial endospore formation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 112 citations in OpenAlex.
- Distinctive spore architecture and developmental biology ofJournal of bacteriology · 2026Article
- Decoding the functional role of the calcium ATPase YloB in microbially induced calcite precipitation and sporulation inMicrobiology (Reading, England) · 2026Article
- Spatiotemporal expression of endospore appendages and cryo-EM insights into Ena1C-mediated S-ENA anchoring in Bacillus paranthracis.Scientific reports · 2026Article
- Developmentally regulated proteolysis by MdfA and ClpCP mediates metabolic differentiation duringGenes & development · 2025Article
- Where and why have so many metabolic enzymes gone from developing spores ofGenes & development · 2025Review
- Deciphering metabolic differentiation during Bacillus subtilis sporulation.Nature communications · 2025Article
- Clostridioides difficile Sporulation.Advances in experimental medicine and biology · 2024Review
- Glutamate catabolism during sporulation determines the success of the future spore germination.iScience · 2022Article
- To Feed or to Stick? Genomic Analysis Offers Clues for the Role of a Molecular Machine in Endospore Formers.Journal of bacteriology · 2022Review
- High Resolution Analysis of Proteome Dynamics duringInternational journal of molecular sciences · 2021Article
- Levels and Characteristics of mRNAs in Spores of Firmicute Species.Journal of bacteriology · 2021Article
- Article
- Distribution and preservation of the components of the engulfment. What is beyond representative genomes?PloS one · 2021Article
- Metabolic differentiation and intercellular nurturing underpin bacterial endospore formation.Science advances · 2021Article
- Milestones inMicrobial cell (Graz, Austria) · 2020Review
- Channels modestly impact compartment-specific ATP levels during Bacillus subtilis sporulation and a rise in the mother cell ATP level is not necessary for Pro-σMolecular microbiology · 2020Article
- Shaping an Endospore: Architectural Transformations DuringAnnual review of microbiology · 2020Review
- Bacterial Spore mRNA - What's Up With That?Frontiers in microbiology · 2020Review
- Single-molecule optical microscopy of protein dynamics and computational analysis of images to determine cell structure development in differentiatingComputational and structural biotechnology journal · 2020Article
- From Root to Tips: Sporulation Evolution and Specialization in Bacillus subtilis and the Intestinal Pathogen Clostridioides difficile.Molecular biology and evolution · 2019Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
At an early stage during Bacillus subtilis endospore development the bacterium divides asymmetrically to produce two daughter cells. The smaller cell (forespore) differentiates into the endospore, while the larger cell (mother cell) becomes a terminally differentiated cell that nurtures the developing forespore. During development the mother cell engulfs the forespore to produce a protoplast, surrounded by two bilayer membranes, which separate it from the cytoplasm of the mother cell. The activation of sigma(G), which drives late gene expression in the forespore, follows forespore engulfment and requires expression of the spoIIIA locus in the mother cell. One of the spoIIIA-encoded proteins SpoIIIAH is targeted specifically to the membrane surrounding the forespore, through an interaction of its C-terminal extracellular domain with the C-terminal extracellular domain of the forespore membrane protein SpoIIQ. We identified a homologous relationship between the C-terminal domain of SpoIIIAH and the YscJ/FliF protein family, members of which form multimeric rings involved in type III secretion systems and flagella. If SpoIIIAH forms a similar ring structure, it may also form a channel between the mother cell and forespore membranes. To test this hypothesis we developed a compartmentalized biotinylation assay, which we used to show that the C-terminal extracellular domain of SpoIIIAH is accessible to enzymatic modification from the forespore cytoplasm. These and other results lead us to suggest that SpoIIIAH forms part of a channel between the forespore and mother cell that is required for the activation of sigma(G).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.