ArticleNucleic acids research2008
Enhanced gene trapping in mouse embryonic stem cells.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- Comparison and Characteristics of MASLD Mouse Models.Biomedicines · 2026Review
- A whole-genome scan for Artemisinin cytotoxicity reveals a novel therapy for human brain tumors.EMBO molecular medicine · 2023Article
- Inducible de novo expression of neoantigens in tumor cells and mice.Nature biotechnology · 2021Article
- Unbiased compound-protein interface mapping and prediction of chemoresistance loci through forward genetics in haploid stem cells.Oncotarget · 2018Article
- New tools for old drugs: Functional genetic screens to optimize current chemotherapy.Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy · 2018Review
- A reversible haploid mouse embryonic stem cell biobank resource for functional genomics.Nature · 2017Article
- Generation of a multipurposeDisease models & mechanisms · 2017Article
- Conditional Creation and Rescue of Nipbl-Deficiency in Mice Reveals Multiple Determinants of Risk for Congenital Heart Defects.PLoS biology · 2016Article
- Generation of hypoxanthine phosphoribosyltransferase gene knockout rabbits by homologous recombination and gene trapping through somatic cell nuclear transfer.Scientific reports · 2015Article
- LoxP-FRT Trap (LOFT): a simple and flexible system for conventional and reversible gene targeting.BMC biology · 2012Article
- Article
- Forward and reverse genetics through derivation of haploid mouse embryonic stem cells.Cell stem cell · 2011Article
- Gene trap mutagenesis in the mouse.Methods in enzymology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene trapping is used to introduce insertional mutations into genes of mouse embryonic stem cells (ESCs). It is performed with gene trap vectors that simultaneously mutate and report the expression of the endogenous gene at the site of insertion and provide a DNA tag for rapid identification of the disrupted gene. Gene traps have been employed worldwide to assemble libraries of mouse ESC lines harboring mutations in single genes, which can be used to make mutant mice. However, most of the employed gene trap vectors require gene expression for reporting a gene trap event and therefore genes that are poorly expressed may be under-represented in the existing libraries. To address this problem, we have developed a novel class of gene trap vectors that can induce gene expression at insertion sites, thereby bypassing the problem of intrinsic poor expression. We show here that the insertion of the osteopontin enhancer into several conventional gene trap vectors significantly increases the gene trapping efficiency in high-throughput screens and facilitates the recovery of poorly expressed genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.