ArticleThe Journal of biological chemistry2008
Tracking cell surface GABAB receptors using an alpha-bungarotoxin tag.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Normalization of network activity in an epilepsy model with a constitutively active GABBR2 variant.Brain : a journal of neurology · 2026Article
- Functional signatures of de novo GABBR1 and GABBR2 variants associated with neurodevelopmental disorders.NPJ genomic medicine · 2026Article
- Postbiotic potential of Lactococcus lactis CNCM I-5388 in alleviating visceral pain in female rat through GABA production: The innovative concept of the "active-GAD bag".FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Sustained Baclofen-Induced Activation of GABAFrontiers in molecular neuroscience · 2021Article
- Dopamine transporter trafficking and Rit2 GTPase: Mechanism of action andThe Journal of biological chemistry · 2020Article
- A novel bungarotoxin binding site-tagged construct reveals MAPK-dependent Kv4.2 trafficking.Molecular and cellular neurosciences · 2019Article
- CaMolecular neurobiology · 2019Article
- Interrogating Synaptic Architecture: Approaches for Labeling Organelles and Cytoskeleton Components.Frontiers in synaptic neuroscience · 2019Review
- Single-molecule labeling for studying trafficking of renal transporters.American journal of physiology. Renal physiology · 2018Review
- Development of a High-Throughput Flow Cytometry Assay to Monitor Defective Trafficking and Rescue of Long QT2 Mutant hERG Channels.Frontiers in physiology · 2018Article
- Desensitized D2 autoreceptors are resistant to trafficking.Scientific reports · 2017Article
- Neuronal Chloride Regulation via KCC2 Is Modulated through a GABAThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Lys-63-linked Ubiquitination of γ-Aminobutyric Acid (GABA), Type B1, at Multiple Sites by the E3 Ligase Mind Bomb-2 Targets GABAB Receptors to Lysosomal Degradation.The Journal of biological chemistry · 2016Article
- LQT1 mutations in KCNQ1 C-terminus assembly domain suppress IKs using different mechanisms.Cardiovascular research · 2014Article
- Transport along the dendritic endoplasmic reticulum mediates the trafficking of GABAB receptors.Journal of cell science · 2014Article
- Using an α-bungarotoxin binding site tag to study GABA A receptor membrane localization and trafficking.Journal of visualized experiments : JoVE · 2014Article
- Differential regulation of GABAB receptor trafficking by different modes of N-methyl-D-aspartate (NMDA) receptor signaling.The Journal of biological chemistry · 2014Article
- GABAA receptor membrane insertion rates are specified by their subunit composition.Molecular and cellular neurosciences · 2013Article
- Up-regulation of GABA(B) receptor signaling by constitutive assembly with the K+ channel tetramerization domain-containing protein 12 (KCTD12).The Journal of biological chemistry · 2013Article
- High frequency stimulation of the subthalamic nucleus leads to presynaptic GABA(B)-dependent depression of subthalamo-nigral afferents.PloS one · 2013Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
GABA(B) receptors mediate slow synaptic inhibition in the central nervous system and are important for synaptic plasticity as well as being implicated in disease. Located at pre- and postsynaptic sites, GABA(B) receptors will influence cell excitability, but their effectiveness in doing so will be dependent, in part, on their trafficking to, and stability on, the cell surface membrane. To examine the dynamic behavior of GABA(B) receptors in GIRK cells and neurons, we have devised a method that is based on tagging the receptor with the binding site components for the neurotoxin, alpha-bungarotoxin. By using the alpha-bungarotoxin binding site-tagged GABA(B) R1a subunit (R1a(BBS)), co-expressed with the R2 subunit, we can track receptor mobility using the small reporter, alpha-bungarotoxin-conjugated rhodamine. In this way, the rates of internalization and membrane insertion for these receptors could be measured with fixed and live cells. The results indicate that GABA(B) receptors rapidly turnover in the cell membrane, with the rate of internalization affected by the state of receptor activation. The bungarotoxin-based method of receptor-tagging seems ideally suited to follow the dynamic regulation of other G-protein-coupled receptors.
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