ArticleAnnals of biomedical engineering2008
Modeling fluorescence recovery after photobleaching in loaded bone: potential applications in measuring fluid and solute transport in the osteocytic lacunar-canalicular system.
Article in Annals of biomedical engineering, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed.
- Predicting cortical bone resorption in the mouse tibia under disuse conditions caused by transient muscle paralysis.Scientific reports · 2025Article
- Mechanical deformations of bone generate interstitial fluid flow at nanoscale velocities around osteocytes.Frontiers in bioengineering and biotechnology · 2025Article
- Derivation, validation, and prediction of loading-induced mineral apposition rates at endocortical and periosteal bone surfaces based on fluid velocity and pore pressure.Bone reports · 2023Article
- Article
- Perspective on optical imaging for functional assessment in musculoskeletal extremity trauma surgery.Journal of biomedical optics · 2020Article
- Network architecture strongly influences the fluid flow pattern through the lacunocanalicular network in human osteons.Biomechanics and modeling in mechanobiology · 2020Article
- Numerical analysis of the flow field in the lacunar-canalicular system under different magnitudes of gravity.Medical & biological engineering & computing · 2020Article
- Solute Transport in the Bone Lacunar-Canalicular System (LCS).Current osteoporosis reports · 2018Review
- Microstructural changes associated with osteoporosis negatively affect loading-induced fluid flow around osteocytes in cortical bone.Journal of biomechanics · 2018Article
- A Perfusion Culture System for Assessing Bone Marrow Stromal Cell Differentiation on PLGA Scaffolds for Bone Repair.Frontiers in bioengineering and biotechnology · 2018Article
- Poromicromechanics reveals that physiological bone strains induce osteocyte-stimulating lacunar pressure.Biomechanics and modeling in mechanobiology · 2016Article
- A multiscale 3D finite element analysis of fluid/solute transport in mechanically loaded bone.Bone research · 2016Article
- Seeing through Musculoskeletal Tissues: Improving In Situ Imaging of Bone and the Lacunar Canalicular System through Optical Clearing.PloS one · 2016Article
- Altered mechanical environment of bone cells in an animal model of short- and long-term osteoporosis.Biophysical journal · 2015Article
- Dynamic permeability of the lacunar-canalicular system in human cortical bone.Biomechanics and modeling in mechanobiology · 2014Article
- Perlecan-containing pericellular matrix regulates solute transport and mechanosensing within the osteocyte lacunar-canalicular system.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2014Article
- FRAP in pharmaceutical research: practical guidelines and applications in drug delivery.Pharmaceutical research · 2014Review
- Quantifying load-induced solute transport and solute-matrix interaction within the osteocyte lacunar-canalicular system.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2013Article
- Advances in assessment of bone porosity, permeability and interstitial fluid flow.Journal of biomechanics · 2013Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Solute transport through the bone lacunar-canalicular system is essential for osteocyte viability and function, and it can be measured using fluorescence recovery after photobleaching (FRAP). The mathematical model developed here aims to analyze solute transport during FRAP in mechanically loaded bone. Combining both whole bone-level poroelasticity and cellular-level solute transport, we found that load-induced solute transport during FRAP is characterized by an exponential recovery rate, which is determined by the dimensionless Strouhal (St) number that characterizes the oscillation effects over the mean flows, and that significant transport occurs only for St values below a threshold, when the solute stroke displacement exceeds the distance between the source and sink (the canalicular length). This threshold mechanism explains the general flow behaviors such as increasing transport with increasing magnitude and decreasing frequency. Mechanical loading is predicted to enhance transport of all tracers relative to diffusion, with the greatest enhancement for medium-sized tracers and less enhancement for small and large tracers. This study provides guidelines for future FRAP experiments, based on which the model can be used to quantify bone permeability, solute-matrix interaction, and flow velocities. These studies should provide insights into bone adaptation and metabolism, and help to treat various bone diseases and conditions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.