ArticleProceedings of the National Academy of Sciences of the United States of America2008
Discovery of posttranslational maturation by self-subunit swapping.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 83 citations in OpenAlex.
- Bacterial Metallostasis: Metal Sensing, Metalloproteome Remodeling, and Metal Trafficking.Chemical reviews · 2024Review
- Microbial degradation mechanisms of the neonicotinoids acetamiprid and flonicamid and the associated toxicity assessments.Frontiers in microbiology · 2024Review
- COG0523 proteins: a functionally diverse family of transition metal-regulated G3E P-loop GTP hydrolases from bacteria to man.Metallomics : integrated biometal science · 2021Article
- The Fe-type nitrile hydratase from Rhodococcus equi TG328-2 forms an alpha-activator protein complex.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2020Article
- Efficient Overproduction of Active Nitrile Hydratase by Coupling Expression Induction and Enzyme MaturationFrontiers in bioengineering and biotechnology · 2020Article
- Maturation Mechanism of Nitrile Hydratase FromFrontiers in microbiology · 2020Article
- Recent Advances and Promises in Nitrile Hydratase: From Mechanism to Industrial Applications.Frontiers in bioengineering and biotechnology · 2020Review
- Advances in cloning, structural and bioremediation aspects of nitrile hydratases.Molecular biology reports · 2019Review
- Analyzing the function of the insert region found between the α and β-subunits in the eukaryotic nitrile hydratase from Monosiga brevicollis.Archives of biochemistry and biophysics · 2018Article
- Amide compound synthesis by adenylation domain of bacillibactin synthetase.The Journal of antibiotics · 2017Article
- Overexpression and characterization of two types of nitrile hydratases from Rhodococcus rhodochrous J1.PloS one · 2017Article
- Successful expression of the Bordetella petrii nitrile hydratase activator P14K and the unnecessary role of Ser115.BMC biotechnology · 2016Article
- Construction of a subunit-fusion nitrile hydratase and discovery of an innovative metal ion transfer pattern.Scientific reports · 2016Article
- Natural low-molecular mass organic compounds with oxidase activity as organocatalysts.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- A new synthetic route to N-benzyl carboxamides through the reverse reaction of N-substituted formamide deformylase.Applied and environmental microbiology · 2014Article
- An aeroplysinin-1 specific nitrile hydratase isolated from the marine sponge Aplysina cavernicola.Marine drugs · 2013Article
- Strategy for successful expression of the Pseudomonas putida nitrile hydratase activator P14K in Escherichia coli.BMC biotechnology · 2013Article
- Sequential oxidations of thiolates and the cobalt metallocenter in a synthetic metallopeptide: implications for the biosynthesis of nitrile hydratase.Inorganic chemistry · 2013Article
- Metallation and mismetallation of iron and manganese proteins in vitro and in vivo: the class I ribonucleotide reductases as a case study.Metallomics : integrated biometal science · 2012Review
- YeiR: a metal-binding GTPase from Escherichia coli involved in metal homeostasis.Metallomics : integrated biometal science · 2012Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several general mechanisms of metallocenter biosynthesis have been reported and reviewed, and in all cases, the components or subunits of an apoprotein remain in the final holoprotein. Here, we first discovered that one subunit of an apoenzyme did not remain in the functional holoenzyme. The cobalt-containing low-molecular-mass nitrile hydratase (L-NHase) of Rhodococcus rhodochrous J1 consists of beta- and alpha-subunits encoded by the nhlBA genes, respectively. An ORF, nhlE, just downstream of nhlBA, was found to be necessary for L-NHase activation. In contrast to the cobalt-containing L-NHase (holo-L-NHase containing Cys-SO(2)(-) and Cys-SO(-) metal ligands) derived from nhlBAE, the gene products derived from nhlBA were cobalt-free L-NHase (apo-L-NHase lacking oxidized cysteine residues). We discovered an L-NHase maturation mediator, NhlAE, consisting of NhlE and the cobalt- and oxidized cysteine-containing alpha-subunit of L-NHase. The incorporation of cobalt into L-NHase was shown to depend on the exchange of the nonmodified cobalt-free alpha-subunit of apo-L-NHase with the cobalt-containing cysteine-modified alpha-subunit of NhlAE. This is a posttranslational maturation process different from general mechanisms of metallocenter biosynthesis known so far: the unexpected behavior of a protein in a protein complex, which we named "self-subunit swapping."
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.