ArticleMolecular and cellular biology2008
MCAK-independent functions of ch-Tog/XMAP215 in microtubule plus-end dynamics.
Article in Molecular and cellular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 68 citations in OpenAlex.
- Article
- Centrosome age breaks spindle size symmetry even in cells thought to divide symmetrically.The Journal of cell biology · 2024Article
- CKAP5 stabilizes CENP-E at kinetochores by regulating microtubule-chromosome attachments.EMBO reports · 2024Article
- Cortical dynein drives centrosome clustering in cells with centrosome amplification.Molecular biology of the cell · 2023Article
- Therapeutic gene silencing ofScience advances · 2023Article
- TACC3-ch-TOG interaction regulates spindle microtubule assembly by controlling centrosomal recruitment of γ-TuRC.Bioscience reports · 2023Article
- Article
- Characterization of a recently synthesized microtubule-targeting compound that disrupts mitotic spindle poles in human cells.Scientific reports · 2021Article
- Article
- A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division.Nature communications · 2020Article
- Targeting centrosome amplification, an Achilles' heel of cancer.Biochemical Society transactions · 2019Review
- Human Mitotic Centromere-Associated Kinesin Is Targeted by MicroRNA 485-5p/181c and Prognosticates Poor Survivability of Breast Cancer.Journal of oncology · 2019Article
- TOG-tubulin binding specificity promotes microtubule dynamics and mitotic spindle formation.The Journal of cell biology · 2017Article
- Cytoskeleton-associated protein 5 and clathrin heavy chain binding regulates spindle assembly in mouse oocytes.Oncotarget · 2017Article
- AURKA, DLGAP5, TPX2, KIF11 and CKAP5: Five specific mitosis-associated genes correlate with poor prognosis for non-small cell lung cancer patients.International journal of oncology · 2017Article
- Upregulation of colonic and hepatic tumor overexpressed gene is significantly associated with the unfavorable prognosis marker of human hepatocellular carcinoma.American journal of cancer research · 2016Article
- A sensitised RNAi screen reveals a ch-TOG genetic interaction network required for spindle assembly.Scientific reports · 2015Article
- TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation.Biology open · 2015Article
- A small compound targeting TACC3 revealed its different spatiotemporal contributions for spindle assembly in cancer cells.Oncogene · 2014Article
- Coordination of adjacent domains mediates TACC3-ch-TOG-clathrin assembly and mitotic spindle binding.The Journal of cell biology · 2013Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
The formation of a functional bipolar mitotic spindle is essential for genetic integrity. In human cells, the microtubule polymerase XMAP215/ch-Tog ensures spindle bipolarity by counteracting the activity of the microtubule-depolymerizing kinesin XKCM1/MCAK. Their antagonistic effects on microtubule polymerization confer dynamic instability on microtubules assembled in cell-free systems. It is, however, unclear if a similar interplay governs microtubule behavior in mammalian cells in vivo. Using real-time analysis of spindle assembly, we found that ch-Tog is required to produce or maintain long centrosomal microtubules after nuclear-envelope breakdown. In the absence of ch-Tog, microtubule assembly at centrosomes was impaired and microtubules were nondynamic. Interkinetochore distances and the lengths of kinetochore fibers were also reduced in these cells. Codepleting MCAK with ch-Tog improved kinetochore fiber length and interkinetochore separation but, surprisingly, did not rescue centrosomal microtubule assembly and microtubule dynamics. Our data therefore suggest that ch-Tog has at least two distinct roles in spindle formation. First, it protects kinetochore microtubules from depolymerization by MCAK. Second, ch-Tog plays an essential role in centrosomal microtubule assembly, a function independent of MCAK activity. Thus, the notion that the antagonistic activities of MCAK and ch-Tog determine overall microtubule stability is too simplistic to apply to human cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.