ArticleJournal of medical microbiology2008
Molecular detection methods and serotyping performed directly on clinical samples improve diagnostic sensitivity and reveal increased incidence of invasive disease by Streptococcus pneumoniae in Italian children.
Article in Journal of medical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers, 1 of them a synthesis that pooled it.
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Who cites it
44 citing papers in PubMed, 1 synthesis or guideline pooled it, 107 citations in OpenAlex.
- PCR using blood for diagnosis of invasive pneumococcal disease: systematic review and meta-analysis.Journal of clinical microbiology · 2010Pooled it
- Real-time qPCR improves meningitis pathogen detection in invasive bacterial-vaccine preventable disease surveillance in Fiji.Scientific reports · 2016Trial
- The Molecular Approaches and Challenges ofPolish journal of microbiology · 2023Review
- [The new 15-valent pneumococcal conjugate vaccine for the prevention ofJournal of preventive medicine and hygiene · 2023Article
- Serotypes and Clonal Composition of Streptococcus pneumoniae Isolates Causing IPD in Children and Adults in Catalonia before 2013 to 2015 and after 2017 to 2019 Systematic Introduction of PCV13.Microbiology spectrum · 2021Article
- Pneumococcal Serotype Identification by Capsular Sequence Typing (CST): A Modified Novel Approach for Serotyping Directly in Clinical Samples.Diagnostics (Basel, Switzerland) · 2021Article
- High-throughput nanofluidic real-time PCR to discriminate Pneumococcal Conjugate Vaccine (PCV)-associated serogroups 6, 18, and 22 to serotypes using modified oligonucleotides.Scientific reports · 2021Article
- Effectiveness of Pneumococcal Conjugate Vaccines Against Community-acquired Alveolar Pneumonia Attributable to Vaccine-serotype Streptococcus pneumoniae Among Children.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2021Article
- Invasive Pneumococcal Disease in Tuscany Region, Italy, 2016-2017: Integrating Multiple Data Sources to Investigate Underreporting.International journal of environmental research and public health · 2020Article
- Clonal diversity of Haemophilus influenzae carriage isolated from under the age of 6 years children.BMC research notes · 2019Article
- The etiology of acute meningitis and encephalitis syndromes in a sentinel pediatric hospital, Shenzhen, China.BMC infectious diseases · 2019Article
- Serotypes & penicillin susceptibility ofThe Indian journal of medical research · 2018Article
- Sequencing of the variable region ofOpen biology · 2017Article
- Standardization of Laboratory Methods for the PERCH Study.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2017Article
- Epidemiology of invasive pneumococcal disease in Saudi Arabian children younger than 5years of age.Journal of epidemiology and global health · 2016Article
- Underestimation of Invasive Meningococcal Disease in Italy.Emerging infectious diseases · 2016Article
- Enhanced Diagnosis of Pneumococcal Bacteremia Using Antigen- and Molecular-Based Tools on Blood Specimens in Mali and Thailand: A Prospective Surveillance Study.The American journal of tropical medicine and hygiene · 2016Article
- How recent advances in molecular tests could impact the diagnosis of pneumonia.Expert review of molecular diagnostics · 2016Review
- The burden of bacteremia and invasive diseases in children aged less than five years with fever in Italy.Italian journal of pediatrics · 2015Article
- Assessing the impact of pneumococcal conjugate vaccines on invasive pneumococcal disease using polymerase chain reaction-based surveillance: an experience from South Africa.BMC infectious diseases · 2015Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aims of this study were to evaluate the incidence of invasive pneumococcal disease (IPD) in Italian children and perform serotyping by PCR-based assays directly on clinical samples. A 1-year paediatric (0-14 years) population-based surveillance study was designed to evaluate the incidence of IPD in the province of Florence, Italy, by cultural and molecular methods. Among 92 children (80 with pneumonia, 8 with meningitis/sepsis, 4 with arthritis), 4 cases of IPD were diagnosed both by culture and real-time PCR and 18 cases exclusively by molecular methods. The sensitivity of molecular methods was significantly higher than that of cultural methods (Cohen's kappa 0.41; McNemar P=0.000008). The incidence of IPD in children below 2 years of age was 11.5/100,000 and 51.8/100,000 by cultural and molecular methods, respectively. Pneumococcal serotyping by multiplex sequential PCR was obtained in 19/22 samples. Real-time PCR and multiplex sequential PCR can be used directly on biological samples, improving the ability to diagnose IPD. The incidence of IPD appears 5-10 times higher by PCR than by cultural methods.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.