ArticleBiochimica et biophysica acta2009
Nuclear factor I transcription factors regulate IGF binding protein 5 gene transcription in human osteoblasts.
Article in Biochimica et biophysica acta, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 31 citations in OpenAlex.
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- Integrative analysis of long non-coding RNA and mRNA in broilers with valgus-varus deformity.PloS one · 2020Article
- LncRNA LINC00461 Promotes Colorectal Cancer Progression via miRNA-323b-3p/NFIB Axis.OncoTargets and therapy · 2019Article
- MBNL splicing activity depends on RNA binding site structural context.Nucleic acids research · 2018Article
- Disruption of mesoderm formation during cardiac differentiation due to developmental exposure to 13-cis-retinoic acid.Scientific reports · 2018Article
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- A Genome-Wide Scan Identifies Variants in NFIB Associated with Metastasis in Patients with Osteosarcoma.Cancer discovery · 2015Article
- miR-124 regulates fetal pulmonary epithelial cell maturation.American journal of physiology. Lung cellular and molecular physiology · 2015Article
- The role of the insulin‑like growth factor (IGF) axis in osteogenic and odontogenic differentiation.Cellular and molecular life sciences : CMLS · 2014Review
- Identification of a neuronal transcription factor network involved in medulloblastoma development.Acta neuropathologica communications · 2013Article
- Pivotal regulatory network and genes in osteosarcoma.Archives of medical science : AMS · 2013Article
- Combined allelic dosage ofBrain and neuroscience advancesArticle
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Insulin-like growth factor binding protein 5 (IGFBP5) is expressed in many cell types including osteoblasts and modulates IGF activities. IGFBP5 may affect osteoblasts and bone formation, in part by mechanisms independent of binding IGFs. The highly conserved IGFBP5 proximal promoter within 100 nucleotides of the start of transcription contains functional cis regulatory elements for C/EBP, Myb and AP-2. We report evidence for a functional Nuclear Factor I (NFI) cis element that mediates activation or repression of IGFBP5 transcription by the NFI gene family. All four NFI genes were expressed in human osteoblast cultures and osteosarcoma cell lines. Co-transfection with human IGFBP5 promoter luciferase reporter and murine Nfi expression vectors showed that Nfib was the most active in stimulating transcription. Nfix was less active and Nfia and Nfic were inhibitory. Knockdown of NFIB and NFIC expression using siRNA decreased and increased IGFBP5 expression, respectively. Analysis of IGFBP5 promoter deletion and mutation reporter constructs identified a functional NFI cis element. All four NFI proteins bound the NFI site in electrophoretic mobility shift experiments and NFIB bound in chromatin immunoprecipitation assays. Results suggest that NFI proteins are important regulators of IGFBP5 expression in human osteoblasts and thus in modulating IGFBP5 functions in bone.
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