ArticleBMC microbiology2008
The stress responsive and morphologically regulated hsp90 gene from Paracoccidioides brasiliensis is essential to cell viability.
Article in BMC microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 43 citations in OpenAlex.
- Heat Shock Proteins in Medically Relevant Fungal Pathogens: From Molecular Chaperones to Virulence Factors and Therapeutic Targets.Journal of fungi (Basel, Switzerland) · 2026Review
- Signaling Pathways Regulating Dimorphism in Medically Relevant Fungal Species.Pathogens (Basel, Switzerland) · 2025Review
- Common virulence factors betweenCurrent research in microbial sciences · 2024Review
- Global Molecular Response ofJournal of fungi (Basel, Switzerland) · 2023Article
- Updates inJournal of fungi (Basel, Switzerland) · 2021Review
- Article
- Argentilactone Molecular Targets in Paracoccidioides brasiliensis Identified by Chemoproteomics.Antimicrobial agents and chemotherapy · 2018Article
- Heat Shock Proteins in Histoplasma and Paracoccidioides.Clinical and vaccine immunology : CVI · 2017Review
- Review
- Differential Gene Expression of Heat Shock Protein 90 (Hsp90) of Candida albicans obtained from Malaysian and Iranian Patients.The Malaysian journal of medical sciences : MJMS · 2016Article
- Heat Shock Proteins in Dermatophytes: Current Advances and Perspectives.Current genomics · 2016Article
- Detrimental Effect of Fungal 60-kDa Heat Shock Protein on Experimental Paracoccidioides brasiliensis Infection.PloS one · 2016Article
- Effects of Argentilactone on the Transcriptional Profile, Cell Wall and Oxidative Stress of Paracoccidioides spp.PLoS neglected tropical diseases · 2016Article
- Role of Heat-Shock Proteins in Cellular Function and in the Biology of Fungi.Biotechnology research international · 2015Review
- Paracoccidioides-host Interaction: An Overview on Recent Advances in the Paracoccidioidomycosis.Frontiers in microbiology · 2015Review
- The heat-induced molecular disaggregase Hsp104 of Candida albicans plays a role in biofilm formation and pathogenicity in a worm infection model.Eukaryotic cell · 2012Article
- Influence of 17β-estradiol on gene expression of Paracoccidioides during mycelia-to-yeast transition.PloS one · 2011Article
- Virtual prototyping study shows increased ATPase activity of Hsp90 to be the key determinant of cancer phenotype.Systems and synthetic biology · 2010Article
- Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundParacoccidioides brasiliensis is a dimorphic fungus that causes the most prevalent systemic mycosis in Latin America. The response to heat shock is involved in pathogenesis, as this pathogen switches from mycelium to yeast forms in a temperature dependent fashion that is essential to establish infection. HSP90 is a molecular chaperone that helps in the folding and stabilization of selected polypeptides. HSP90 family members have been shown to present important roles in fungi, especially in the pathogenic species, as an immunodominant antigen and also as a potential antifungal therapeutic target.
resultsIn this work, we decided to further study the Pbhsp90 gene, its expression and role in cell viability because it plays important roles in fungal physiology and pathogenesis. Thus, we have sequenced a Pbhsp90 cDNA and shown that this gene is present on the genome as a single copy. We have also confirmed its preferential expression in the yeast phase and its overexpression during dimorphic transition and oxidative stress. Treatment of the yeast with the specific HSP90 inhibitors geldanamycin and radicicol inhibited growth at 2 and 10 microM, respectively.
conclusionThe data confirm that the Pbhsp90 gene encodes a morphologically regulated and stress-responsive protein whose function is essential to cell viability of this pathogen. This work also enforces the potential of HSP90 as a target for antifungal therapies, since the use of HSP90 inhibitors is lethal to the P. brasiliensis yeast cells in a dose-responsive manner.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.