ArticleParasites & vectors2008
Localisation of laminin within Plasmodium berghei oocysts and the midgut epithelial cells of Anopheles stephensi.
Article in Parasites & vectors, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Targeting the mosquito prefoldin-chaperonin complex blocks Plasmodium transmission.Nature microbiology · 2025Article
- Plasmodium GPI-anchored micronemal antigen is essential for parasite transmission through the mosquito host.Molecular microbiology · 2024Article
- Plasmodium sporozoite excystation involves local breakdown of the oocyst capsule.Scientific reports · 2023Article
- The α-Gal Syndrome and Potential Mechanisms.Frontiers in allergy · 2021Review
- NPC1161B, an 8-Aminoquinoline Analog, Is Metabolized in the Mosquito and InhibitsFrontiers in pharmacology · 2019Article
- PfCap380 as a marker for Plasmodium falciparum oocyst development in vivo and in vitro.Malaria journal · 2018Article
- Bioluminescence Method forAntimicrobial agents and chemotherapy · 2017Article
- Optimization of a Membrane Feeding Assay for Plasmodium vivax Infection in Anopheles albimanus.PLoS neglected tropical diseases · 2016Article
- Basement membrane and cell integrity of self-tissues in maintaining Drosophila immunological tolerance.PLoS genetics · 2014Article
- Injury and immune response: applying the danger theory to mosquitoes.Frontiers in plant science · 2014Article
- No evidence for positive selection at two potential targets for malaria transmission-blocking vaccines in Anopheles gambiae s.s.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2013Article
- Malaria parasite-synthesized heme is essential in the mosquito and liver stages and complements host heme in the blood stages of infection.PLoS pathogens · 2013Article
- An impossible journey? The development of Plasmodium falciparum NF54 in Culex quinquefasciatus.PloS one · 2013Article
- Article
- A new role for an old antimicrobial: lysozyme c-1 can function to protect malaria parasites in Anopheles mosquitoes.PloS one · 2011Article
- Mosquito cell line glycoproteins: an unsuitable model system for the Plasmodium ookinete-mosquito midgut interaction?Parasites & vectors · 2010Article
- Article
- Malaria parasite development in the mosquito and infection of the mammalian host.Annual review of microbiology · 2009Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
unlabelled
backgroundOocysts of the malaria parasite form and develop in close proximity to the mosquito midgut basal lamina and it has been proposed that components of this structure play a crucial role in the development and maturation of oocysts that produce infective sporozoites. It is further suggested that oocysts incorporate basal lamina proteins into their capsule and that this provides them with a means to evade recognition by the mosquito's immune system. The site of production of basal lamina proteins in insects is controversial and it is still unclear whether haemocytes or midgut epithelial cells are the main source of components of the mosquito midgut basal lamina. Of the multiple molecules that compose the basal lamina, laminin is known to interact with a number of Plasmodium proteins. In this study, the localisation of mosquito laminin within the capsule and cytoplasm of Plasmodium berghei oocysts and in the midgut epithelial cells of Anopheles stephensi was investigated.
resultsAn ultrastructural examination of midgut sections from infected and uninfected An. stephensi was performed. Post-embedded immunogold labelling demonstrated the presence of laminin within the mosquito basal lamina. Laminin was also detected on the outer surface of the oocyst capsule, incorporated within the capsule and associated with sporozoites forming within the oocysts. Laminin was also found within cells of the midgut epithelium, providing support for the hypothesis that these cells contribute towards the formation of the midgut basal lamina.
conclusionWe suggest that ookinetes may become coated in laminin as they pass through the midgut epithelium. Thereafter, laminin secreted by midgut epithelial cells and/or haemocytes, binds to the outer surface of the oocyst capsule and that some passes through and is incorporated into the developing oocysts. The localisation of laminin on sporozoites was unexpected and the importance of this observation is less clear.
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Registered trials
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