ArticleInflammation2008
Glutamine reduces TNF-alpha by enhancing glutathione synthesis in lipopolysaccharide-stimulated alveolar epithelial cells of rats.
Article in Inflammation, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Nanovesicles for Sensitive Skin Care Developed via Self-Assembly of Glutamine Linoleate.Journal of cosmetic dermatology · 2025Trial
- The role of glutamine metabolism in the pathogenesis of idiopathic pulmonary fibrosis and its therapeutic potential.Frontiers in oncology · 2026Review
- Dietary α-ketoglutarate alleviates glycinin and β-conglycinin induced damage in the intestine of mirror carp (Frontiers in immunology · 2023Article
- Redox regulation of immunity and the role of small molecular weight thiols.Redox biology · 2021Article
- An Overview of Nrf2 Signaling Pathway and Its Role in Inflammation.Molecules (Basel, Switzerland) · 2020Review
- GSH-C4 Acts as Anti-inflammatory Drug in Different Models of Canonical and Cell Autonomous Inflammation Through NFκB Inhibition.Frontiers in immunology · 2019Article
- Amino acids as modulators of the European seabass, Dicentrarchus labrax, innate immune response: an in vitro approach.Scientific reports · 2017Article
- Article
- The protective effects of glutamine in a rat model of ventilator-induced lung injury.Journal of thoracic disease · 2014Article
- Article
- Differential response of primary alveolar type I and type II cells to LPS stimulation.PloS one · 2013Article
- LPS-stimulated cytokine production in type I cells is modulated by the renin-angiotensin system.American journal of respiratory cell and molecular biology · 2012Article
- Glutathione supplementation attenuates lipopolysaccharide-induced mitochondrial dysfunction and apoptosis in a mouse model of acute lung injury.Frontiers in physiology · 2012Article
- Oral glutamine attenuates cyclophosphamide-induced oxidative stress in the bladder but does not prevent hemorrhagic cystitis in rats.Journal of medical toxicology : official journal of the American College of Medical Toxicology · 2011Article
- Aging adversely affects the cigarette smoke-induced glutathione adaptive response in the lung.American journal of respiratory and critical care medicine · 2010Article
- Isothiocyanates induce oxidative stress and suppress the metastasis potential of human non-small cell lung cancer cells.BMC cancer · 2010Article
- Nrf2-Keap1 signaling as a potential target for chemoprevention of inflammation-associated carcinogenesis.Pharmaceutical research · 2010Review
- The Role of Nrf2 in Cellular Innate Immune Response to Inflammatory Injury.Toxicological research · 2009Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To investigate the role of glutathione (GSH) synthesis in the regulation on nuclear factor (NF)-kappaB activity and tumor necrosis factor-alpha (TNF-alpha) release by glutamine (GLN) in lipopolysaccharide (LPS)-stimulated alveolar type II (AT-II) epithelial cells of rat lungs. Primary cultured AT-II cells were pre-treated with various doses of GLN for 2, 8, 16, 24 h. At the 8 h time point before LPS stimulation, various doses of L: -buthionine-(S,R)-sulfoximine (BSO), an inhibitor of GSH synthesis, were added with 10 mM GLN. Then the cells were stimulated with 1 mug/ml LPS for 24 h. The cells were obtained for GSH measurement. TNF-alpha level in the supernatant was determined by enzyme-linked immunosorbent assay. NF-kappaB activity was assessed by electrophoretic mobility shift assay. Eight hours before LPS exposure was the best time point for GLN's enhancing GSH synthesis. LPS could significantly decrease the GSH level, increase NF-kappaB activation and TNF-alpha release in AT-II cells. Supplementation of GLN could increase the GSH level and attenuate the release of TNF-alpha in LPS-stimulated AT-II cells in a dose-dependant manner. And NF-kappaB activation also could be prevented by GLN. BSO could block the effect of GLN. As a precursor of GSH, glutamine could prevent the NF-kappaB activation and attenuate the release of TNF-alpha in LPS-stimulated AT-II cells and the effect may be mediated via GSH synthesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.