ArticleMedical molecular morphology2008
Immunohistochemical and electron microscopic characterization of brush cells of the rat cecum.
Article in Medical molecular morphology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 13 citations in OpenAlex.
- Modulation of the Altered Intestinal Microbiota by Use of Antibiotics with a Novel Synbiotic on Wistar Rats.Probiotics and antimicrobial proteins · 2025Article
- Tuft cells are key mediators of interkingdom interactions at mucosal barrier surfaces.PLoS pathogens · 2022Review
- Novel protocol to observe the intestinal tuft cell using transmission electron microscopy.Biology open · 2022Article
- The Immune Function of Tuft Cells at Gut Mucosal Surfaces and Beyond.Journal of immunology (Baltimore, Md. : 1950) · 2019Review
- Interpreting heterogeneity in intestinal tuft cell structure and function.The Journal of clinical investigation · 2018Review
- Carbonic anhydrase II binds to and increases the activity of the epithelial sodium-proton exchanger, NHE3.American journal of physiology. Renal physiology · 2015Article
- The Na⁺/H⁺ exchanger isoform 3 is required for active paracellular and transcellular Ca²⁺ transport across murine cecum.American journal of physiology. Gastrointestinal and liver physiology · 2013Article
- The divergence, actions, roles, and relatives of sodium-coupled bicarbonate transporters.Physiological reviews · 2013Review
- Quantitative distribution of brush cells in the rat gastrointestinal tract: brush cell population coincides with NaHCO3 secretion.Medical molecular morphology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brush cells (BCs) are relatively rare cells that are sparsely distributed throughout the mammalian digestive and respiratory systems. BCs have been identified in the rodent large intestine, but these cells have not been characterized by immunocytochemistry or electron microscopy. We previously demonstrated that rat bile duct BCs had strong immunoreactivity for six proteins that function in HCO(3)(-) secretion and thus assumed that BCs secrete NaHCO(3). It is well known that the gastrointestinal (GI) tract secretes NaHCO(3), but it is not known whether BCs of the GI tract also express proteins related to HCO(3)(-) secretion. Thus, in the present study, using double immunostaining for cytokeratin 18, a specific marker for BCs, we investigated protein expression in BCs from the rodent GI tract. We show that BCs from the GI tract express six proteins related to HCO(3)(-) secretion: cystic fibrosis transmembrane conductance regulator (CFTR), Cl(-)/HCO(3)(-) exchanger, Na(+)/HCO(3)(-) cotransporter, carbonic anhydrase II, Na(+)/H(+) exchanger (NHE) 1, and NHE3. These results suggest that BCs from the GI tract secrete NaHCO(3). In addition, we examined BCs from the rat cecum using electron microscopy (EM). Transmission EM (TEM) showed that BCs have long microvilli, a well-developed tubulovesicular system, and an abundant cytoskeleton. Scanning EM revealed that BCs were scattered on the luminal surface of the cecum and had numerous long microvilli.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.