ArticleHistochemistry and cell biology2009
Spiny versus stubby: 3D reconstruction of human myenteric (type I) neurons.
Article in Histochemistry and cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed.
- Classification of human enteric neurons.Histochemistry and cell biology · 2021Review
- Chagasic megacolon: enteric neurons and related structures.Histochemistry and cell biology · 2014Review
- What neurons hide behind calretinin immunoreactivity in the human gut?Histochemistry and cell biology · 2014Article
- Article
- Three-dimensional image quantification as a new morphometry method for tissue engineering.Tissue engineering. Part C, Methods · 2012Article
- Visualization of image data from cells to organisms.Nature methods · 2010Review
- Extending the knowledge in histochemistry and cell biology.Histochemistry and cell biology · 2010Review
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Authors and funding
7 authors.
Funding
Abstract
We have compared the three-dimensional (3D) morphology of stubby and spiny neurons derived from the human small intestine. After immunohistochemical triple staining for leu-enkephalin (ENK), vasoactive intestinal peptide (VIP) and neurofilament (NF), neurons were selected and scanned based on their immunoreactivity, whether ENK (stubby) or VIP (spiny). For the 3D reconstruction, we focused on confocal data pre-processing with intensity drop correction, non-blind deconvolution, an additional compression procedure in z-direction, and optimizing segmentation reliability. 3D Slicer software enabled a semi-automated segmentation based on an objective threshold (interrater and intrarater reliability, both 0.99). We found that most dendrites of stubby neurons emerged only from the somal circumference, whereas in spiny neurons, they also emerged from the luminal somal surface. In most neurons, the nucleus was positioned abluminally in its soma. The volumes of spiny neurons were significantly larger than those of stubby neurons (total mean of stubbies 806 +/- 128 mum(3), of spinies 2,316 +/- 545 mum(3)), and spiny neurons had more dendrites (26.3 vs. 11.3). The ratios of somal versus dendritic volumes were 1:1.2 in spiny and 1:0.3 in stubby neurons. In conclusion, 3D reconstruction revealed new differences between stubby and spiny neurons and allowed estimations of volumetric data of these neuron populations.
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