Evidence map›Paper›PMID 18806992›Full record

ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2008

Bin mapping of genomic and EST-derived SSRs in melon (Cucumis melo L.).

I Fernandez-Silva, I Eduardo, J Blanca, C Esteras, B Picó, F Nuez, P Arús, J Garcia-Mas, Antonio José Monforte

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Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.

0numbers the graph read from it
0cells of the map it votes in
45citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

45 citing papers in PubMed.

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  5. Frontiers in genetics · 2020
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  10. Frontiers in plant science · 2017
    Article
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  14. Article
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  18. Article
  19. SNP genotyping in melons: genetic variation, population structure, and linkage disequilibrium.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2013
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

I Fernandez-SilvaIRTA, Centre de Recerca en Agrigenòmica CSIC-IRTA-UAB (CRAG), Carretera de Cabrils, Km 2, 08348, Cabrils, Barcelona, Spain.
I Eduardo
J Blanca
C Esteras
B Picó
F Nuez
P Arús
J Garcia-Mas
Antonio José Monforte

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We report the development of 158 primer pairs flanking SSR motifs in genomic (gSSR) and EST (EST-SSR) melon sequences, all yielding polymorphic bands in melon germplasm, except one that was polymorphic only in Cucurbita species. A similar polymorphism level was found among EST-SSRs and gSSRs, between dimeric and trimeric EST-SSRs, and between EST-SSRs placed in the open reading frame or any of the 5'- or 3'-untranslated regions. Correlation between SSR length and polymorphism was only found for dinucleotide EST-SSRs located within the untranslated regions, but not for trinucleotide EST-SSRs. Transferability of EST-SSRs to Cucurbita species was assayed and 12.7% of the primer pairs amplified at least in one species, although only 5.4% were polymorphic. A set of 14 double haploid lines from the cross between the cultivar "Piel de Sapo" and the accession PI161375 were selected for the bin mapping approach in melon. One hundred and twenty-one SSR markers were newly mapped. The position of 46 SSR loci was also verified by genotyping the complete population. A final bin-map was constructed including 80 RFLPs, 212 SSRs, 3 SNPs and the Nsv locus, distributed in 122 bins with an average bin length of 10.2 cM and a maximum bin length of 33 cM. Map density was 4.2 cM/marker or 5.9 cM/SSR.

Indexed as

Chromosome MappingExpressed Sequence TagsMinisatellite RepeatsPolymorphism, GeneticCucumis meloDNA, PlantDNA PrimersGenome, PlantGenotypeSequence Analysis, DNADNA, PlantDNA Primers

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.