ArticleApplied and environmental microbiology2008
Global transcription and metabolic flux analysis of Escherichia coli in glucose-limited fed-batch cultivations.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- A redox-sensitive phosphatase regulates glycolysis as a metabolic switch in the bacterial inner membrane.Science advances · 2026Article
- A General Process-Based Model for Describing the Metabolic Shift in Microbial Cell Cultures.Frontiers in microbiology · 2020Article
- GREACE-assisted adaptive laboratory evolution in endpoint fermentation broth enhances lysine production by Escherichia coli.Microbial cell factories · 2019Article
- Influence of Glucose Availability and CRP Acetylation on the Genome-Wide Transcriptional Response ofFrontiers in microbiology · 2018Article
- In Silico Prediction of Large-Scale Microbial Production Performance: Constraints for Getting Proper Data-Driven Models.Computational and structural biotechnology journal · 2018Review
- Cell-free protein synthesis from non-growing, stressed Escherichia coli.Scientific reports · 2017Article
- The FBPase Encoding Gene glpX Is Required for Gluconeogenesis, Bacterial Proliferation and Division In Vivo of Mycobacterium marinum.PloS one · 2016Article
- Quantitative constraint-based computational model of tumor-to-stroma coupling via lactate shuttle.Scientific reports · 2015Article
- CeCaFDB: a curated database for the documentation, visualization and comparative analysis of central carbon metabolic flux distributions explored by 13C-fluxomics.Nucleic acids research · 2015Article
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- Global transcriptome and physiological responses of Acinetobacter oleivorans DR1 exposed to distinct classes of antibiotics.PloS one · 2014Article
- Self-inducible Bacillus subtilis expression system for reliable and inexpensive protein production by high-cell-density fermentation.Applied and environmental microbiology · 2011Article
- Effect of iclR and arcA knockouts on biomass formation and metabolic fluxes in Escherichia coli K12 and its implications on understanding the metabolism of Escherichia coli BL21 (DE3).BMC microbiology · 2011Article
- Novel members of the Cra regulon involved in carbon metabolism in Escherichia coli.Journal of bacteriology · 2011Article
- Novel roles of cAMP receptor protein (CRP) in regulation of transport and metabolism of carbon sources.PloS one · 2011Article
- Metabolomic and transcriptomic stress response of Escherichia coli.Molecular systems biology · 2010Article
- Bridging the gap between fluxomics and industrial biotechnology.Journal of biomedicine & biotechnology · 2010Review
- Teaching bioprocess engineering to undergraduates: Multidisciplinary hands-on training in a one-week practical course.Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular BiologyArticle
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Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A time series of whole-genome transcription profiling of Escherichia coli K-12 W3110 was performed during a carbon-limited fed-batch process. The application of a constant feed rate led to the identification of a dynamic sequence of diverse carbon limitation responses (e.g., the hunger response) and at the same time provided a global view of how cellular and extracellular resources are used: the synthesis of high-affinity transporters guarantees maximal glucose influx, thereby preserving the phosphoenolpyruvate pool, and energy-dependent chemotaxis is reduced in order to provide a more economic "work mode." sigma(S)-mediated stress and starvation responses were both found to be of only minor relevance. Thus, the experimental setup provided access to the hunger response and enabled the differentiation of the hunger response from the general starvation response. Our previous topological model of the global regulation of the E. coli central carbon metabolism through the crp, cra, and relA/spoT modulons is supported by correlating transcript levels and metabolic fluxes and can now be extended. The substrate is extensively oxidized in the tricarboxylic acid (TCA) cycle to enhance energy generation. However, the general rate of oxidative decarboxylation within the pentose phosphate pathway and the TCA cycle is restricted to a minimum. Fine regulation of the carbon flux through these pathways supplies sufficient precursors for biosyntheses. The pools of at least three precursors are probably regulated through activation of the (phosphoenolpyruvate-)glyoxylate shunt. The present work shows that detailed understanding of the genetic regulation of bacterial metabolism provides useful insights for manipulating the carbon flux in technical production processes.
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