ArticleApplied and environmental microbiology2008
Effective mutagenesis of Vibrio fischeri by using hyperactive mini-Tn5 derivatives.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Functional genomics of chitin degradation by Vibrio parahaemolyticus reveals finely integrated metabolic contributions to support environmental fitness.PLoS genetics · 2025Article
- Transient infection ofApplied and environmental microbiology · 2024Article
- Expanding the capabilities of MuGENT for large-scale genetic engineering of the fastest-replicating species,Microbiology spectrum · 2024Article
- A prototrophic suppressor of aJournal of bacteriology · 2024Article
- A Rapid Fluorescence-Based Screen to Identify Regulators and Components of Interbacterial Competition Mechanisms in Bacteria.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Activation of the Type VI Secretion System in the Squid SymbiontJournal of bacteriology · 2021Article
- A lasting symbiosis: how Vibrio fischeri finds a squid partner and persists within its natural host.Nature reviews. Microbiology · 2021Review
- Polyunsaturated fatty acids cause physiological and behavioral changes in Vibrio alginolyticus and Vibrio fischeri.MicrobiologyOpen · 2021Article
- Vibrio fischeri Amidase Activity Is Required for Normal Cell Division, Motility, and Symbiotic Competence.Applied and environmental microbiology · 2021Article
- Identification of a novel bacterial receptor that binds tail tubular proteins and mediates phage infection ofEmerging microbes & infections · 2020Article
- Genetic Manipulation of Vibrio fischeri.Current protocols in microbiology · 2020Article
- Vibrio fischeri siderophore production drives competitive exclusion during dual-species growth.Molecular microbiology · 2020Article
- The regulatory network of Vibrio parahaemolyticus type VI secretion system 1.Environmental microbiology · 2019Article
- Nitric oxide inhibits biofilm formation by Vibrio fischeri via the nitric oxide sensor HnoX.Molecular microbiology · 2019Article
- Generation and In Vivo Characterization of Tn5-Induced Biofilm Mutants of Vibrio cholerae O139.Current microbiology · 2018Article
- Vibrio fischeri Biofilm Formation Prevented by a Trio of Regulators.Applied and environmental microbiology · 2018Article
- The Transcriptional Regulator HlyU Positively Regulates Expression ofJournal of bacteriology · 2018Article
- Tools for Rapid Genetic Engineering of Vibrio fischeri.Applied and environmental microbiology · 2018Article
- Article
- An Expanded Transposon Mutant Library Reveals that Vibrio fischeri δ-Aminolevulinate Auxotrophs Can Colonize Euprymna scolopes.Applied and environmental microbiology · 2017Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
We have developed a transposon mutagenesis system for Vibrio fischeri ES114 that utilizes a hyperactive mutant Tn5 transposase (E54K and M56A) and optimized transposon ends. Using a conjugation-based procedure, we obtained independent single-insertion mini-Tn5 mutants at a rate of approximately 10(-6). This simple and inexpensive technique represents a significant improvement over previous methods for transposon mutagenesis of V. fischeri and should be applicable to many other bacteria.
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