ArticleJournal of bacteriology2008
Mutations in conserved helix 69 of 23S rRNA of Thermus thermophilus that affect capreomycin resistance but not posttranscriptional modifications.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 19 citations in OpenAlex.
- Microevolutionary models and multi-omics analysis uncover the cross-drug resistance characteristics of capreomycin-selectedCurrent research in microbial sciences · 2025Article
- Tuberactinomycin antibiotics: Biosynthesis, anti-mycobacterial action, and mechanisms of resistance.Frontiers in microbiology · 2022Review
- Cryo-electron microscopy structure of the 70S ribosome from Enterococcus faecalis.Scientific reports · 2020Article
- The structural basis for inhibition of ribosomal translocation by viomycin.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- The development of peptide ligands that target helix 69 rRNA of bacterial ribosomes.Bioorganic & medicinal chemistry · 2016Article
- Molecular mechanism of viomycin inhibition of peptide elongation in bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- The antituberculosis antibiotic capreomycin inhibits protein synthesis by disrupting interaction between ribosomal proteins L12 and L10.Antimicrobial agents and chemotherapy · 2014Article
- Tools for characterizing bacterial protein synthesis inhibitors.Antimicrobial agents and chemotherapy · 2013Article
- Increased bacterial hemolytic activity is conferred by expression of TlyA methyltransferase but not by its 2'-O-methylation of the ribosome.Current microbiology · 2013Article
- Capreomycin susceptibility is increased by TlyA-directed 2'-O-methylation on both ribosomal subunits.Molecular microbiology · 2012Article
- Comparison of solution conformations and stabilities of modified helix 69 rRNA analogs from bacteria and human.Biopolymers · 2012Article
- Molecular basis for the selectivity of antituberculosis compounds capreomycin and viomycin.Antimicrobial agents and chemotherapy · 2011Article
- The structures of the anti-tuberculosis antibiotics viomycin and capreomycin bound to the 70S ribosome.Nature structural & molecular biology · 2010Article
- Helix 69 in 23S rRNA modulates decoding by wild type and suppressor tRNAs.Molecular genetics and genomics : MGG · 2009Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
Abstract
Translocation during the elongation phase of protein synthesis involves the relative movement of the 30S and 50S ribosomal subunits. This movement is the target of tuberactinomycin antibiotics. Here, we describe the isolation and characterization of mutants of Thermus thermophilus selected for resistance to the tuberactinomycin antibiotic capreomycin. Two base substitutions, A1913U and mU1915G, and a single base deletion, DeltamU1915, were identified in helix 69 of 23S rRNA, a structural element that forms part of an interribosomal subunit bridge with the decoding center of 16S rRNA, the site of previously reported capreomycin resistance base substitutions. Capreomycin resistance in other bacteria has been shown to result from inactivation of the TlyA methyltransferase which 2'-O methylates C1920 of 23S rRNA. Inactivation of the tlyA gene in T. thermophilus does not affect its sensitivity to capreomycin. Finally, none of the mutations in helix 69 interferes with methylation at C1920 or with pseudouridylation at positions 1911 and 1917. We conclude that the resistance phenotype is a consequence of structural changes introduced by the mutations.
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