ArticleBiophysical journal2008
Alternative versions of the myosin relay domain differentially respond to load to influence Drosophila muscle kinetics.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed.
- Drosophila myosin function and muscle structure are modulated by isoform-specific amino acid residues of the relay domain.Biochemical and biophysical research communications · 2026Article
- Structure of the Inhibited Smooth Muscle Myosin and Its Implications on the Regulation of Insect Striated Muscle Myosin.Life (Basel, Switzerland) · 2025Review
- A Drosophila cardiac myosin increases jump muscle stretch activation and shortening deactivation.Biophysical journal · 2025Article
- X-ray Crystallographic and Molecular Dynamic Analyses of Drosophila melanogaster Embryonic Muscle Myosin Define Domains Responsible for Isoform-Specific Properties.Journal of molecular biology · 2020Article
- The load dependence of muscle's force-velocity curve is modulated by alternative myosin converter domains.American journal of physiology. Cell physiology · 2019Article
- Five Alternative Myosin Converter Domains Influence Muscle Power, Stretch Activation, and Kinetics.Biophysical journal · 2018Article
- The Relay/Converter Interface Influences Hydrolysis of ATP by Skeletal Muscle Myosin II.The Journal of biological chemistry · 2016Article
- The roles of troponin C isoforms in the mechanical function of Drosophila indirect flight muscle.Journal of muscle research and cell motility · 2014Article
- A new experimental model to study force depression: the Drosophila jump muscle.Journal of applied physiology (Bethesda, Md. : 1985) · 2014Article
- Mapping interactions between myosin relay and converter domains that power muscle function.The Journal of biological chemistry · 2014Article
- An embryonic myosin converter domain influences Drosophila indirect flight muscle stretch activation, power generation and flight.The Journal of experimental biology · 2014Article
- An embryonic myosin isoform enables stretch activation and cyclical power in Drosophila jump muscle.Biophysical journal · 2013Article
- Calcium signalling indicates bilateral power balancing in the Drosophila flight muscle during manoeuvring flight.Journal of the Royal Society, Interface · 2013Article
- Alternative relay and converter domains tune native muscle myosin isoform function in Drosophila.Journal of molecular biology · 2012Article
- Transgenic expression and purification of myosin isoforms using the Drosophila melanogaster indirect flight muscle system.Methods (San Diego, Calif.) · 2012Article
- Mechanical analysis of Drosophila indirect flight and jump muscles.Methods (San Diego, Calif.) · 2012Review
- Disrupting the myosin converter-relay interface impairs Drosophila indirect flight muscle performance.Biophysical journal · 2011Article
- Nucleotide pocket thermodynamics measured by EPR reveal how energy partitioning relates myosin speed to efficiency.Journal of molecular biology · 2011Article
- Chronic heart failure decreases cross-bridge kinetics in single skeletal muscle fibres from humans.The Journal of physiology · 2010Article
- The influence of myosin converter and relay domains on cross-bridge kinetics of Drosophila indirect flight muscle.Biophysical journal · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
We measured the influence of alternative versions of the Drosophila melanogaster myosin heavy chain relay domain on muscle mechanical properties. We exchanged relay domain regions (encoded by alternative versions of exon 9) between an embryonic (EMB) isoform and the indirect flight muscle isoform (IFI) of myosin. Previously, we observed no effect of exchanging the EMB relay domain region into the flight muscle isoform (IFI-9b) on in vitro actin motility velocity or solution ATPase measurements compared to IFI. However, in indirect flight muscle fibers, IFI-9b exhibited decreased maximum power generation (P(max)) and optimal frequency of power generation (f(max)) to 70% and 83% of IFI fiber values. The decrease in muscle performance reduced the flight ability and wing-beat frequency of IFI-9b Drosophila compared to IFI Drosophila. Previously, we found that exchanging the flight muscle specific relay domain into the EMB isoform (EMB-9a) prevented actin movement in the in vitro motility assay compared to EMB, which does support actin movement. However, in indirect flight muscle fibers EMB-9a was a highly effective motor, increasing P(max) and f(max) 2.5-fold and 1.4-fold, respectively, compared to fibers expressing EMB. We propose that the oscillatory load EMB-9a experiences in the muscle fiber reduces a high activation energy barrier between two strongly bound states of the cross-bridge cycle, thereby promoting cross-bridge cycling. The IFI relay domain's enhanced sensitivity to load increases cross-bridge kinetics, whereas the EMB version is less load-sensitive.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.