Evidence map›Paper›PMID 18804446›Full record

ArticleArchives of biochemistry and biophysics2008

Spectroscopic and kinetic studies of Nor1, a cytochrome P450 nitric oxide reductase from the fungal pathogen Histoplasma capsulatum.

Lily Y Chao, Jasper Rine, Michael A Marletta

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Archives of biochemistry and biophysics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.5field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 17 citations in OpenAlex.

  1. Histoplasma Capsulatum: Mechanisms for Pathogenesis.Current topics in microbiology and immunology · 2019
    Review
  2. Article
  3. Review
  4. Fungal traits that drive ecosystem dynamics on land.Microbiology and molecular biology reviews : MMBR · 2015
    Review
  5. Article
  6. Article
  7. mSphere
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Lily Y ChaoDepartment of Plant and Microbial Biology, University of California, Berkeley, CA, USA.
Jasper Rine
Michael A Marletta
University of California, Berkeley · US

Funding

GENETIC ANALYSIS OF THE GENES CONTROLLING A POSITIONR01GM031105 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI RINE, JASPER D · 1985 to 2020
$6.4M
GENETIC ANALYSIS OF GENES CONTROLLING A POSITION EFFECTR37GM031105 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI RINE, JASPER D · 1999 to 2008
$3.5M
REGULATION OF THE STEROL BIOSYNTHETIC PATHWAY IN YEASTR01GM035827 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI RINE, JASPER D · 1986 to 2005
$2.0M
NIGMS NIH HHS GM31105NIGMS NIH HHS GM35827NIGMS NIH HHS R01 GM031105NIGMS NIH HHS R01 GM035827NIGMS NIH HHS R37 GM031105
6 · The paper itself

Abstract

The fungal respiratory pathogen Histoplasma capsulatum evades the innate immune response and colonizes macrophages during infection. Although macrophage production of the antimicrobial effector nitric oxide (NO) restricts H. capsulatum growth, the pathogen is able to establish a persistent infection. H. capsulatum contains a P450 nitric oxide reductase homologue (NOR1) that may be important for detoxifying NO during infection. To characterize the activity of this putative P450 enzyme, a 404 amino acid fragment of Nor1p was expressed in Escherichia coli and purified to homogeneity. Spectral characterization of Nor1p indicated that it was similar to other fungal P450 nitric oxide reductases. Nor1p catalyzed the reduction of NO to N2O using NADH as the direct reductant. The K(M) for NO was determined to be 20 microM and the k(cat) to be 5000 min(-1). Together, these results provide evidence for a protective role of a P450 nitric oxide reductase against macrophage-derived NO.

Indexed as

Amino Acid SequenceEscherichia coliHemoglobinsHistoplasmaKineticsMembrane Transport ProteinsModels, ChemicalMolecular Sequence DataNADPH-Ferrihemoprotein ReductaseNitric OxideOxidation-ReductionOxidoreductasesPlasmidsSaccharomyces cerevisiae ProteinsSequence Homology, Amino AcidSpectrophotometryHemoglobinsMembrane Transport ProteinsNADPH-Ferrihemoprotein ReductaseNitric Oxidenitric-oxide reductaseOxidoreductasesSaccharomyces cerevisiae ProteinsSGE1 protein, S cerevisiaeTranscription Factors

Identifiers

PMID18804446
PMCPMC2603478
OpenAlexW2053193845

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.