ArticleBMC microbiology2008
Development of a real-time multiplex PCR assay for the detection of multiple Salmonella serotypes in chicken samples.
Article in BMC microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Prevalence and risk factors for Salmonella infection in layer flocks in Israel between 2018 and 2023.Poultry science · 2026Article
- High-throughput multiplex qPCR for distinguishing Salmonella serovars Heidelberg, Enteritidis, Typhimurium, Gallinarum, and Pullorum.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Identification of Salmonella enterica biovars Gallinarum and Pullorum and their antibiotic resistance pattern in integrated crop-livestock farms and poultry meats.Access microbiology · 2024Article
- Virulence and Antimicrobial-Resistant Gene Profiles ofInternational journal of microbiology · 2024Article
- A Multiplex PCR Method for Simultaneous Detection of Infectious Laryngotracheitis Virus andVeterinary sciences · 2023Article
- Assessment of the prevalence, serotype, and antibiotic resistance pattern ofFrontiers in microbiology · 2023Article
- Evaluation of the different methods to detect Salmonella in poultry feces samples.Archives of microbiology · 2022Article
- Isolation of Salmonella spp. from black spiny-tailed iguana (Ctenosaura similis) meat commercialised in markets of León city, Nicaragua.Veterinary medicine and science · 2022Article
- Molecular detection ofIranian journal of veterinary research · 2022Article
- Prevalence, virulence factor and antimicrobial resistance analysis of Salmonella Enteritidis from poultry and egg samples in Iran.BMC veterinary research · 2021Article
- Thymol Nanoemulsion: A New Therapeutic Option for Extensively Drug Resistant Foodborne Pathogens.Antibiotics (Basel, Switzerland) · 2020Article
- Molecular identification and antibiotic resistance profiling of Salmonella species isolated from chickens in eastern Turkey.BMC veterinary research · 2020Article
- Molecular Detection of Multidrug ResistantPathogens (Basel, Switzerland) · 2020Article
- Article
- Occurrence ofItalian journal of food safety · 2019Article
- Molecular Tools To Study Preharvest Food Safety Challenges.Microbiology spectrum · 2018Review
- Screening and DetectingFrontiers in microbiology · 2017Article
- Epidemiological, molecular characterization and antibiotic resistance ofGut pathogens · 2017Article
- A Novel Pretreatment-Free Duplex Chamber Digital PCR Detection System for the Absolute Quantitation of GMO Samples.International journal of molecular sciences · 2016Article
- Thermal inactivation of Salmonella Enteritidis on chicken skin previously exposed to acidified Sodium chlorite or tri-sodium phosphate.Journal of food science and technology · 2015Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundA real-time multiplex PCR assay was developed for the detection of multiple Salmonella serotypes in chicken samples. Poultry-associated serotypes detected in the assay include Enteritidis, Gallinarum, Typhimurium, Kentucky and Dublin. The traditional cultural method according to EN ISO 6579:2002 for the detection of Salmonella in food was performed in parallel. The real-time PCR based method comprised a pre-enrichment step in Buffered Peptone Water (BPW) overnight, followed by a shortened selective enrichment in Rappaport Vasilliadis Soya Broth (RVS) for 6 hours and subsequent DNA extraction.
resultsThe real-time multiplex PCR assay and traditional cultural method showed 100% inclusivity and 100% exclusivity on all strains tested. The real-time multiplex PCR assay was as sensitive as the traditional cultural method in detecting Salmonella in artificially contaminated chicken samples and correctly identified the serotype. Artificially contaminated chicken samples resulted in a detection limit of between 1 and 10 CFU per 25 g sample for both methods. A total of sixty-three naturally contaminated chicken samples were investigated by both methods and relative accuracy, relative sensitivity and relative specificity of the real-time PCR method were determined to be 89, 94 and 87%, respectively. Thirty cultures blind tested were correctly identified by the real-time multiplex PCR method.
conclusionReal-time PCR methodology can contribute to meet the need for rapid identification and detection methods in food testing laboratories.
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