ArticleJournal of immunology (Baltimore, Md. : 1950)2008
The Pseudomonas toxin pyocyanin inhibits the dual oxidase-based antimicrobial system as it imposes oxidative stress on airway epithelial cells.
Article in Journal of immunology (Baltimore, Md. : 1950), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
70 citing papers in PubMed, 117 citations in OpenAlex.
- Efficacy of 1% acetic acid in the treatment of chronic wounds infected with Pseudomonas aeruginosa: prospective randomised controlled clinical trial.International wound journal · 2016Trial
- A phase II study on safety and efficacy of high-dose N-acetylcysteine in patients with cystic fibrosis.European journal of medical research · 2009Trial
- Determinants of susceptibility and tolerance ofInfection and immunity · 2026Review
- Factors governing the response ofFrontiers in cellular and infection microbiology · 2026Review
- Modulation of pulmonary immune functions by theFrontiers in immunology · 2025Review
- Synthesis of polypyrrole/cellulose nanocrystals disks for removal of pyocyanin metabolite biomarker released by Pseudomonas aeruginosa.PloS one · 2025Article
- Flagellum-deficientMicrobiology spectrum · 2024Article
- The therapeutic potential of thiocyanate and hypothiocyanous acid against pulmonary infections.Free radical biology & medicine · 2024Review
- Article
- Dual oxidase 1 is dispensable duringFrontiers in immunology · 2023Article
- The structure-function relationship of Pseudomonas aeruginosa in infections and its influence on the microenvironment.FEMS microbiology reviews · 2022Review
- Development of a Versatile, Low-Cost Electrochemical System to Study Biofilm Redox Activity at the Micron Scale.Applied and environmental microbiology · 2022Article
- Mutation ofAntimicrobial agents and chemotherapy · 2022Article
- Proteobacteria and Firmicutes Secreted Factors Exert Distinct Effects onMetabolites · 2022Article
- The Hypothiocyanite and Amantadine Combination Treatment Prevents Lethal Influenza A Virus Infection in Mice.Frontiers in immunology · 2022Article
- The Role of Oxidative Stress in Sarcoidosis.International journal of molecular sciences · 2021Review
- Dual oxidase 1 promotes antiviral innate immunity.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- DUOX1 in mammalian disease pathophysiology.Journal of molecular medicine (Berlin, Germany) · 2021Review
- Enhancement of pyocyanin production by subinhibitory concentration of royal jelly inF1000Research · 2021Article
- Nlrx1-Regulated Defense and Metabolic Responses toFrontiers in immunology · 2021Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
The dual oxidase-thiocyanate-lactoperoxidase (Duox/SCN(-)/LPO) system generates the microbicidal oxidant hypothiocyanite in the airway surface liquid by using LPO, thiocyanate, and Duox-derived hydrogen peroxide released from the apical surface of the airway epithelium. This system is effective against several microorganisms that infect airways of cystic fibrosis and other immunocompromised patients. We show herein that exposure of airway epithelial cells to Pseudomonas aeruginosa obtained from long-term cultures inhibits Duox1-dependent hydrogen peroxide release, suggesting that some microbial factor suppresses Duox activity. These inhibitory effects are not seen with the pyocyanin-deficient P. aeruginosa strain PA14 Phz1/2. We show that purified pyocyanin, a redox-active virulence factor produced by P. aeruginosa, inhibits human airway cell Duox activity by depleting intracellular stores of NADPH, as it generates intracellular superoxide. Long-term exposure of human airway (primary normal human bronchial and NCI-H292) cells to pyocyanin also blocks induction of Duox1 by Th2 cytokines (IL-4, IL-13), which was prevented by the antioxidants glutathione and N-acetylcysteine. Furthermore, we showed that low concentrations of pyocyanin blocked killing of wild-type P. aeruginosa by the Duox/SCN(-)/LPO system on primary normal human bronchial epithelial cells. Thus, pyocyanin can subvert Pseudomonas killing by the Duox-based system as it imposes oxidative stress on the host. We also show that lactoperoxidase can oxidize pyocyanin, thereby diminishing its cytotoxicity. These data establish a novel role for pyocyanin in the survival of P. aeruginosa in human airways through competitive redox-based reactions between the pathogen and host.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.