ArticleStem cells (Dayton, Ohio)2008
Secreted proteoglycans directly mediate human embryonic stem cell-basic fibroblast growth factor 2 interactions critical for proliferation.
Article in Stem cells (Dayton, Ohio), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 46 citations in OpenAlex.
- Glycosylation in Stem Cell Biology.Handbook of experimental pharmacology · 2025Review
- Cell Surface and Functional Features of Cortical Bone Stem Cells.International journal of molecular sciences · 2021Article
- Integral Roles of Specific Proteoglycans in Hair Growth and Hair Loss: Mechanisms behind the Bioactivity of Proteoglycan Replacement Therapy with Nourkrin® with Marilex® in Pattern Hair Loss and Telogen Effluvium.Dermatology research and practice · 2020Review
- Developments in cell culture systems for human pluripotent stem cells.World journal of stem cells · 2019Review
- Osteogenic differentiation enhances the MC3T3-E1 secretion of glycosaminoglycans with an affinity for basic fibroblast growth factor and bone morphogenetic protein-2.Regenerative therapy · 2018Article
- Mouse embryonic fibroblast (MEF)/BMP4-conditioned medium enhanced multipotency of human dental pulp cells.Journal of molecular histology · 2018Article
- An Alternative Method for Long-Term Culture of Chicken Embryonic Stem CellStem cells international · 2018Article
- Article
- Self-renewal and cell lineage differentiation strategies in human embryonic stem cells and induced pluripotent stem cells.Expert opinion on biological therapy · 2014Review
- Fibroblast growth factor signaling is essential for self-renewal of dental epithelial stem cells.The Journal of biological chemistry · 2013Article
- Heparan sulfate: a key regulator of embryonic stem cell fate.Biological chemistry · 2013Review
- Engineering cell-material interfaces for long-term expansion of human pluripotent stem cells.Biomaterials · 2013Article
- Heparan sulfate biosynthesis enzymes in embryonic stem cell biology.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2012Review
- Thermal stability of fibroblast growth factor protein is a determinant factor in regulating self-renewal, differentiation, and reprogramming in human pluripotent stem cells.Stem cells (Dayton, Ohio) · 2012Article
- Combinatorial screening of chemically defined human mesenchymal stem cell culture substrates.Journal of materials chemistry · 2012Article
- Moving from static to dynamic complexity in hydrogel design.Nature communications · 2012Article
- Surfaces that sequester serum-borne heparin amplify growth factor activity.Advanced materials (Deerfield Beach, Fla.) · 2011Article
- Chemically well-defined self-assembled monolayers for cell culture: toward mimicking the natural ECM.Soft matter · 2011Article
- Harnessing endogenous growth factor activity modulates stem cell behavior.Integrative biology : quantitative biosciences from nano to macro · 2011Article
- Biomaterials that regulate growth factor activity via bioinspired interactions.Advanced functional materials · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Human embryonic stem (ES) cells can be maintained in an undifferentiated state if the culture medium is first conditioned on a layer of mouse embryonic fibroblast (MEF) feeder cells. Here we show that human ES cell proliferation is coordinated by MEF-secreted heparan sulfate proteoglycans (HSPG) in conditioned medium (CM). These HSPG and other heparinoids can stabilize basic fibroblast growth factor (FGF2) in unconditioned medium at levels comparable to those observed in CM. They also directly mediate binding of FGF2 to the human ES cell surface, and their removal from CM impairs proliferation. Finally, we have developed a purification scheme for MEF-secreted HSPG in CM. Using column chromatography, immunoblotting, and mass spectrometry-based proteomic analysis, we have identified multiple HSPG species in CM. The results demonstrate that HSPG are key signaling cofactors in CM-based human ES cell culture.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.