ArticleNeonatology2009
Archived unfrozen neonatal blood spots are amenable to quantitative gene expression analysis.
Article in Neonatology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
- The Unique Utility of Newborn Dried Blood Spots for Driving Discovery in Childhood Cancer Research.Clinical chemistry · 2025Review
- Preparation and processing of dried blood spots for microRNA sequencing.Biology methods & protocols · 2023Review
- The Prenatal Origin of Childhood Leukemia: Potential Applications for Epidemiology and Newborn Screening.Frontiers in pediatrics · 2021Review
- Little to Give, Much to Gain-What Can You Do With a Dried Blood Spot?Current environmental health reports · 2020Review
- Dried Blood Spot RNA Transcriptomes Correlate with Transcriptomes Derived from Whole Blood RNA.The American journal of tropical medicine and hygiene · 2018Article
- Optimization of extraction of genomic DNA from archived dried blood spot (DBS): potential application in epidemiological research & bio banking.Gates open research · 2018Article
- Article
- Effect of storage time on gene expression data acquired from unfrozen archived newborn blood spots.Molecular genetics and metabolism · 2016Article
- Psychological resilience and the gene regulatory impact of posttraumatic stress in Nepali child soldiers.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Public concerns regarding the storage and secondary uses of residual newborn bloodspots: an analysis of print media, legal cases, and public engagement activities.Journal of community genetics · 2015Article
- Comparison of frozen and unfrozen blood spots for gene expression studies.The Journal of pediatrics · 2014Article
- High correlations in gene expression between paired umbilical cord blood and neonatal blood of healthy newborns on Guthrie cards.The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians · 2013Article
- Gene expression in archived newborn blood spots distinguishes infants who will later develop cerebral palsy from matched controls.Pediatric research · 2013Article
- A reliable and effective method of DNA isolation from old human blood paper cards.SpringerPlus · 2013Article
- Evaluation of sex-specific gene expression in archived dried blood spots (DBS).International journal of molecular sciences · 2012Article
- Article
- Robustness of genome-wide scanning using archived dried blood spot samples as a DNA source.BMC genetics · 2011Article
- Storage and use of residual newborn screening blood spots: a public policy emergency.Genetics in medicine : official journal of the American College of Medical Genetics · 2011Article
- Cord blood genomic analysis highlights the role of redox balance.Free radical biology & medicine · 2010Article
- Maximizing deoxyribonucleic acid yield from dried blood spots.Journal of diabetes science and technology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundState laws in the USA mandate that blood be drawn from all newborn infants to screen for health-threatening conditions. These screening assays consume only a small portion of the blood samples, which are collected on filter paper ('Guthrie') cards. Many states archive unused blood spots, often in unrefrigerated storage.
objectivesWhile individual RNA transcripts have been identified from archived neonatal blood spots, no study to date has performed quantitative analysis of archived blood spot RNA.
methodsWe demonstrate that RNA can be isolated and amplified from newborn blood spots stored unfrozen for as long as 9 years, and can be analyzed by microarray and qPCR.
resultsMicroarray assays of archived neonatal blood spots consistently detected 3,000-4,000 expressed genes with correlations of 0.90 between replicates. Blood spot mRNA is amenable to qPCR and we detected biologically relevant expression levels of housekeeping and immune-mediating genes.
conclusionsThese experiments demonstrate the feasibility of using blood spots as a source of RNA which can be analyzed using quantitative microarray and qPCR assays. The application of these methods to the analysis of widely collected biological specimens may be a valuable resource for the study of perinatal determinants of disease development.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.