ArticleAmerican journal of physiology. Cell physiology2008
Primary granule exocytosis in human neutrophils is regulated by Rac-dependent actin remodeling.
Article in American journal of physiology. Cell physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 112 citations in OpenAlex.
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- Lysyl Oxidase Promotes Actin-Dependent Neutrophil Activation and Cytotoxicity Toward Retinal Endothelial Cells in Diabetes.Diabetes · 2026Article
- Neutrophil Killing ofJournal of innate immunity · 2026Article
- Cortical Actin Depolymerisation in 3D Cell Culture Enhances Extracellular Vesicle Secretion and Therapeutic Effects.Journal of extracellular vesicles · 2025Article
- miRNA-132-5p mediates a negative feedback regulation of IL-8 secretion through S100A8/A9 downregulation in neutrophil-like HL-60 cells.Frontiers in immunology · 2023Article
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- New Application of the Commercially Available Dye Celestine Blue B as a Sensitive and Selective Fluorescent "Turn-On" Probe for Endogenous Detection of HOCl and Reactive Halogenated Species.Antioxidants (Basel, Switzerland) · 2022Article
- Sex-Based Differences in Human Neutrophil Chemorepulsion.Journal of immunology (Baltimore, Md. : 1950) · 2022Article
- Roles of neutrophil granule proteins in orchestrating inflammation and immunity.The FEBS journal · 2022Review
- Lysophosphatidylcholine induces azurophil granule translocationThe Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2022Article
- CD44/ERM/F-actin complex mediates targeted nuclear degranulation and excessive neutrophil extracellular trap formation during sepsis.Journal of cellular and molecular medicine · 2022Article
- Cytonemes Versus Neutrophil Extracellular Traps in the Fight of Neutrophils with Microbes.International journal of molecular sciences · 2020Review
- The Small GTPase Cdc42 Is a Major Regulator of Neutrophil Effector Functions.Frontiers in immunology · 2020Article
- Inhibition of Neutrophil Primary Granule Release during Yersinia pestis Pulmonary Infection.mBio · 2019Article
- Rho and Rac, but not ROCK, are required for secretion of human and mouse eosinophil-associated RNases.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2019Article
- Neutrophils in Psoriasis.Frontiers in immunology · 2019Review
- Different Isoforms of the Neuronal Guidance Molecule Slit2 Directly Cause Chemoattraction or Chemorepulsion of Human Neutrophils.Journal of immunology (Baltimore, Md. : 1950) · 2019Article
- Ultrastructural changes in peripheral blood leukocytes in α-synuclein knockout mice.Blood cells, molecules & diseases · 2018Article
- Membrane Diffusion Occurs by Continuous-Time Random Walk Sustained by Vesicular Trafficking.Biophysical journal · 2018Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The actin cytoskeleton regulates exocytosis in all secretory cells. In neutrophils, Rac2 GTPase has been shown to control primary (azurophilic) granule exocytosis. In this report, we propose that Rac2 is required for actin cytoskeletal remodeling to promote primary granule exocytosis. Treatment of neutrophils with low doses (< or = 10 microM) of the actin-depolymerizing drugs latrunculin B (Lat B) or cytochalasin B (CB) enhanced both formyl peptide receptor- and Ca(2+) ionophore-stimulated exocytosis. Higher concentrations of CB or Lat B, or stabilization of F-actin with jasplakinolide (JP), inhibited primary granule exocytosis measured as myeloperoxidase release but did not affect secondary granule exocytosis determined by lactoferrin release. These results suggest an obligatory role for F-actin disassembly before primary granule exocytosis. However, lysates from secretagogue-stimulated neutrophils showed enhanced actin polymerization activity in vitro. Microscopic analysis showed that resting neutrophils contain significant cortical F-actin, which was redistributed to sites of primary granule translocation when stimulated. Exocytosis and actin remodeling was highly polarized when cells were primed with CB; however, polarization was reduced by Lat B preincubation, and both polarization and exocytosis were blocked when F-actin was stabilized with JP. Treatment of cells with the small molecule Rac inhibitor NSC23766 also inhibited actin remodeling and primary granule exocytosis induced by Lat B/fMLF or CB/fMLF, but not by Ca(2+) ionophore. Therefore, we propose a role for F-actin depolymerization at the cell cortex coupled with Rac-dependent F-actin polymerization in the cell cytoplasm to promote primary granule exocytosis.
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