ArticleMolecular biology of the cell2008
Retrograde flow and myosin II activity within the leading cell edge deliver F-actin to the lamella to seed the formation of graded polarity actomyosin II filament bundles in migrating fibroblasts.
Article in Molecular biology of the cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
55 citing papers in PubMed, 82 citations in OpenAlex.
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- Phosphorylation of the small heat shock protein HspB1 regulates cytoskeletal recruitment and cell motility.Molecular biology of the cell · 2022Article
- Micron-scale supramolecular myosin arrays help mediate cytoskeletal assembly at mature adherens junctions.The Journal of cell biology · 2022Article
- Non-muscle myosin II and the plasticity of 3D cell migration.Frontiers in cell and developmental biology · 2022Review
- ERK signaling for cell migration and invasion.Frontiers in molecular biosciences · 2022Review
- Lens Fibrosis: Understanding the Dynamics of Cell Adhesion Signaling in Lens Epithelial-Mesenchymal Transition.Frontiers in cell and developmental biology · 2022Review
- Active cytoskeletal composites display emergent tunable contractility and restructuring.Soft matter · 2021Article
- Recursive feedback between matrix dissipation and chemo-mechanical signaling drives oscillatory growth of cancer cell invadopodia.Cell reports · 2021Article
- Cytoskeletal Dynamics in Epithelial-Mesenchymal Transition: Insights into Therapeutic Targets for Cancer Metastasis.Cancers · 2021Review
- Chemokine-biased robust self-organizing polarization of migrating cells in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Neuronal NADPH oxidase 2 regulates growth cone guidance downstream of slit2/robo2.Developmental neurobiology · 2021Article
- Following the footprints of variability during filopodial growth.European biophysics journal : EBJ · 2020Article
- Biomechanical Aspects of Actin Bundle Dynamics.Frontiers in cell and developmental biology · 2020Article
- The focal adhesion scaffold protein Hic-5 regulates vimentin organization in fibroblasts.Molecular biology of the cell · 2019Article
- p90 ribosomal S6 kinase (RSK) phosphorylates myosin phosphatase and thereby controls edge dynamics during cell migration.The Journal of biological chemistry · 2019Article
- The Cytoskeleton-A Complex Interacting Meshwork.Cells · 2019Review
- Ultrastructure of the actin cytoskeleton.Current opinion in cell biology · 2018Review
- Repellent and Attractant Guidance Cues Initiate Cell Migration by Distinct Rear-Driven and Front-Driven Cytoskeletal Mechanisms.Current biology : CB · 2018Article
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3 authors at 1 institution in 1 country.
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Abstract
In migrating fibroblasts actomyosin II bundles are graded polarity (GP) bundles, a distinct organization to stress fibers. GP bundles are important for powering cell migration, yet have an unknown mechanism of formation. Electron microscopy and the fate of photobleached marks show actin filaments undergoing retrograde flow in filopodia, and the lamellipodium are structurally and dynamically linked with stationary GP bundles within the lamella. An individual filopodium initially protrudes, but then becomes separated from the tip of the lamellipodium and seeds the formation of a new GP bundle within the lamella. In individual live cells expressing both GFP-myosin II and RFP-actin, myosin II puncta localize to the base of an individual filopodium an average 28 s before the filopodium seeds the formation of a new GP bundle. Associated myosin II is stationary with respect to the substratum in new GP bundles. Inhibition of myosin II motor activity in live cells blocks appearance of new GP bundles in the lamella, without inhibition of cell protrusion in the same timescale. We conclude retrograde F-actin flow and myosin II activity within the leading cell edge delivers F-actin to the lamella to seed the formation of new GP bundles.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.