ArticleMolecular biology of the cell2008
cAMP-mediated induction of cyclin E sensitizes growth-arrested adipose stem cells to DNA damage-induced apoptosis.
Article in Molecular biology of the cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- The expression and function of miR-424 in infantile skin hemangioma and its mechanism.Scientific reports · 2017Trial
- Mitochondrial Abundant Heat Soluble (MAHS) Protein Expression Modulates Metabolic Dynamics in Human Adipose-Derived Stem Cells.International journal of molecular sciences · 2026Article
- A Review on Peripheral Tinnitus, Causes, and Treatments from the Perspective of Autophagy.Experimental neurobiology · 2022Review
- Deletion of FGF9 in GABAergic neurons causes epilepsy.Cell death & disease · 2021Article
- Effect of cAMP Signaling Regulation in Osteogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells.Cells · 2020Article
- The regulatory role of microRNAs in angiogenesis-related diseases.Journal of cellular and molecular medicine · 2018Review
- A Role for cAMP and Protein Kinase A in Experimental Necrotizing Enterocolitis.The American journal of pathology · 2017Article
- AKAP95 promotes cell cycle progression via interactions with cyclin E and low molecular weight cyclin E.American journal of translational research · 2016Article
- The role of high cell density in the promotion of neuroendocrine transdifferentiation of prostate cancer cells.Molecular cancer · 2014Article
- Cyclin E controls Drosophila female germline stem cell maintenance independently of its role in proliferation by modulating responsiveness to niche signals.Development (Cambridge, England) · 2013Article
- Tightrope act: autophagy in stem cell renewal, differentiation, proliferation, and aging.Cellular and molecular life sciences : CMLS · 2013Review
- Cyclic AMP is both a pro-apoptotic and anti-apoptotic second messenger.Acta physiologica (Oxford, England) · 2012Review
- Plasmin overcomes resistance to prostaglandin E2 in fibrotic lung fibroblasts by reorganizing protein kinase A signaling.The Journal of biological chemistry · 2011Article
- Article
- Caffeine inhibits the viability and osteogenic differentiation of rat bone marrow-derived mesenchymal stromal cells.British journal of pharmacology · 2010Article
- Differential gene expression in adipose stem cells cultured in allogeneic human serum versus fetal bovine serum.Tissue engineering. Part A · 2010Article
- A viral microRNA down-regulates multiple cell cycle genes through mRNA 5'UTRs.PLoS pathogens · 2010Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The differentiation capacity of mesenchymal stem cells has been extensively studied, but little is known on cell cycle-related events in the proliferation and differentiation phases of these cells. Here, we demonstrate that exposure to cAMP-increasing agents inhibits proliferation of adipose stem cells (ASCs). This antiproliferative effect is associated with both reduced cdk2 activity and pRB phosphorylation. Concomitantly, however, the level of cyclin E markedly increases upon cAMP induction, indicating that cyclin E may have cdk2-independent functions in these cells besides its role as a cdk2 activator. Indeed, we found indications of a cdk2-independent role of cyclin E in DNA damage-induced apoptosis. 8-CPT-cAMP sensitizes ASCs to gamma-irradiation-induced apoptosis, an effect abolished by knockdown of cyclin E. Moreover, cAMP induces early activation of ERK, leading to reduced degradation of cyclin E. The cAMP-mediated up-regulation of cyclin E was blocked by knockdown of ERK or by an inhibitor of the ERK kinase MEK. We conclude that cAMP inhibits cdk2 activity and pRB phosphorylation, leading to reduced ASC proliferation. Concomitant with this growth inhibition, however, cyclin E levels are increased in a MEK/ERK-dependent manner. Our results suggest that cyclin E plays an important, cdk2-independent role in genotoxic stress-induced apoptosis in mesenchymal stem cells.
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