ArticlePLoS biology2008
A genome-wide RNAi screen to dissect centriole duplication and centrosome maturation in Drosophila.
Article in PLoS biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 150 papers.
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Who cites it
150 citing papers in PubMed, 238 citations in OpenAlex.
- Unraveling the molecular details of centriole elongation.Trends open · 2026Article
- Phosphorylation remodels the mitotic centrosome matrix to generate bipartite γ-tubulin complex docking sites.Science advances · 2026Article
- Phosphorylation remodels the mitotic centrosome matrix to generate bipartite γ-tubulin complex docking sites.bioRxiv : the preprint server for biology · 2025Article
- A conserved role for centriolar satellites in translation of centrosomal and ciliary proteins.The Journal of cell biology · 2025Article
- In vitro reconstitution of a minimal human centrosome scaffold capable of forming and clustering microtubule asters.Journal of cell science · 2025Article
- Article
- Catalytic growth in a shared enzyme pool ensures robust control of centrosome size.bioRxiv : the preprint server for biology · 2024Article
- CEP192 localises mitotic Aurora-A activity by priming its interaction with TPX2.The EMBO journal · 2024Article
- Cep57 regulates human centrosomes through multivalent interactions.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- γ-TuRCs and the augmin complex are required for the development of highly branched dendritic arbors in Drosophila.Journal of cell science · 2024Article
- Article
- Regulation of centrosome size by the cell-cycle oscillator in Drosophila embryos.The EMBO journal · 2024Article
- Ana1/CEP295 is an essential player in the centrosome maintenance program regulated by Polo kinase and the PCM.EMBO reports · 2024Article
- Centrosomal organization of Cep152 provides flexibility in Plk4 and procentriole positioning.The Journal of cell biology · 2023Article
- Article
- Multifaceted modes of γ-tubulin complex recruitment and microtubule nucleation at mitotic centrosomes.The Journal of cell biology · 2023Article
- Viruses in LaboratoryViruses · 2023Article
- APC/C-dependent degradation of Spd2 regulates centrosome asymmetry in Drosophila neural stem cells.EMBO reports · 2023Article
- Bld10p/Cep135 determines the number of triplets in the centriole independently of the cartwheel.The EMBO journal · 2022Article
- Centriole distal-end proteins CP110 and Cep97 influence centriole cartwheel growth at the proximal end.Journal of cell science · 2022Article
90 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Centrosomes comprise a pair of centrioles surrounded by an amorphous pericentriolar material (PCM). Here, we have performed a microscopy-based genome-wide RNA interference (RNAi) screen in Drosophila cells to identify proteins required for centriole duplication and mitotic PCM recruitment. We analysed 92% of the Drosophila genome (13,059 genes) and identified 32 genes involved in centrosome function. An extensive series of secondary screens classified these genes into four categories: (1) nine are required for centriole duplication, (2) 11 are required for centrosome maturation, (3) nine are required for both functions, and (4) three genes regulate centrosome separation. These 32 hits include several new centrosomal components, some of which have human homologs. In addition, we find that the individual depletion of only two proteins, Polo and Centrosomin (Cnn) can completely block centrosome maturation. Cnn is phosphorylated during mitosis in a Polo-dependent manner, suggesting that the Polo-dependent phosphorylation of Cnn initiates centrosome maturation in flies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.