ArticleHuman genetics1991
Labeling of the centromeric region on human chromosome 8 by in situ hybridization.
Article in Human genetics, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 45 citations in OpenAlex.
- Full Karyotype Interphase Cell Analysis.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2018Article
- High Performance DNA Probes for Perinatal Detection of Numerical Chromosome Aberrations.Advanced techniques in biology & medicine · 2015Article
- Bioinformatics tools allow targeted selection of chromosome enumeration probes and aneuploidy detection.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2013Article
- Chromosome-specific DNA repeats: rapid identification in silico and validation using fluorescence in situ hybridization.International journal of molecular sciences · 2012Article
- Bioinformatic Tools Identify Chromosome-Specific DNA Probes and Facilitate Risk Assessment by Detecting Aneusomies in Extra-embryonic Tissues.Current genomics · 2012Article
- Article
- Somatic genomic variations in extra-embryonic tissues.Current genomics · 2010Article
- Chromosome-specific DNA repeat probes.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2006Article
- Female-specific features of recombinational double-stranded DNA repair in relation to synapsis and telomere dynamics in human oocytes.Chromosoma · 2004Article
- Spatial organization of active and inactive genes and noncoding DNA within chromosome territories.The Journal of cell biology · 2002Article
- Multilocus genetic analysis of single interphase cells by spectral imaging.Human genetics · 2000Article
- Patient-specific probes for preimplantation genetic diagnosis of structural and numerical aberrations in interphase cells.Journal of assisted reproduction and genetics · 1999Article
- Human artificial chromosomes generated by modification of a yeast artificial chromosome containing both human alpha satellite and single-copy DNA sequences.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Molecular characterization of a 130-kb terminal microdeletion at 22q in a child with mild mental retardation.American journal of human genetics · 1997Article
- The fragile X mental retardation syndrome protein interacts with novel homologs FXR1 and FXR2.The EMBO journal · 1995Article
- Isolation and identification of a novel tandemly repeated DNA sequence in the centromeric region of human chromosome 8.Chromosoma · 1993Article
- Article
- Stable karyotypes in epithelial cancer cell lines despite high rates of ongoing structural and numerical chromosomal instability.Neoplasia (New York, N.Y.)Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Probe DNA that binds preferentially to the centromeric region of human chromosomes 8 was synthesized. Alpha satellite probe DNA molecules were selectively amplified from sorter-purified human chromosomes 8 by in vitro DNA amplification using the polymerase chain reaction (PCR). Probe labeling was performed during PCR by incorporation of biotinylated deoxyuridine. In situ hybridization of unpurified probe DNA comprised of alpha satellite monomer and higher molecular weight DNA fragments with metaphase chromosome spreads showed binding to the centromeric regions of numerous chromosomes. However, blocking with unlabeled total human alphoid DNA dramatically reduced crosshybridization to chromosomes other than 8. Under these conditions, the degenerate probe DNA allowed unambiguous visualization of domains occupied by centromeric DNA of chromosome 8 in metaphase spreads and interphase cell nuclei, thus greatly facilitating the detection of numerical chromosome aberrations in tumor cells. In situ hybridization of size-fractionated alpha satellite DNA identified the monomeric fraction as the major cause of crosshybridization. Alpha satellite dimers and higher molecular weight DNA fragments showed relatively high specificity for human chromosomes 8.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.