ArticleProtoplasma2008
Diffusion of anionic and neutral GFP derivatives through plasmodesmata in epidermal cells of Nicotiana benthamiana.
Article in Protoplasma, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- The influence of electrical charge on plasmodesma conductivity.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Distributing Plant Developmental Regulatory Proteins via Plasmodesmata.Plants (Basel, Switzerland) · 2024Review
- Intercellular trafficking via plasmodesmata: molecular layers of complexity.Cellular and molecular life sciences : CMLS · 2021Review
- Similarities and Differences in the GFP Movement in the Zygotic and Somatic Embryos of Arabidopsis.Frontiers in plant science · 2021Article
- Review
- Confinement-Free Wide-Field Ratiometric Tracking of Single Fluorescent Molecules.Biophysical journal · 2019Article
- From plasmodesma geometry to effective symplasmic permeability through biophysical modelling.eLife · 2019Article
- PlaMoM: a comprehensive database compiles plant mobile macromolecules.Nucleic acids research · 2017Article
- Lost in Transit: Long-Distance Trafficking and Phloem Unloading of Protein Signals in Arabidopsis Homografts.The Plant cell · 2016Article
- A model system for analyzing intercellular communication through plasmodesmata using moss protonemata and leaves.Journal of plant research · 2015Review
- Quantitative imaging of directional transport through plasmodesmata in moss protonemata via single-cell photoconversion of Dendra2.Journal of plant research · 2013Article
- Modeling the parameters for plasmodesmal sugar filtering in active symplasmic phloem loaders.Frontiers in plant science · 2013Article
- Rapid and cost-effective fabrication of selectively permeable calcium-alginate microfluidic device using "modified" embedded template method.Biomicrofluidics · 2012Article
- Reduced levels of class 1 reversibly glycosylated polypeptide increase intercellular transport via plasmodesmata.Plant signaling & behavior · 2012Article
- Solute diffusion is hindered in the mitochondrial matrix.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plasmodesmata (Pd) are trans-wall membrane channels that permit cell-to-cell transport of metabolites and other small molecules, proteins, RNAs, and signaling molecules. The transport of cytoplasmic soluble macromolecules is a function of the electrochemical gradient between adjacent cells, the number of Pd per interface between adjacent cells, Stokes radius (R(S)), area of the cytoplasmic annulus, and channel length. The size of the largest molecule that can pass through Pd defines the Pd size exclusion limit. However, since the shape and size of a molecule determines its capacity to diffuse through pores or tubes, R(S) is a better measure. Relatively small changes in R(S) can cause large differences in the mobility of molecular probes, particularly if the pore size is close to that of the probe. In addition, as the dimensions of a macromolecule approach that of the channel, membrane charge effects may become important. We employed quantitative tools and molecular modeling to measure the apparent coefficient of conductivity of Pd, C(Pd), for the non-targeted transport of macromolecules. This method allowed us to examine the influence of protein charge and R(S) on C(Pd) in Nicotiana benthamiana. The C(Pd) of modified green fluorescent proteins (GFPs) of different sizes but with the same charge as native GFP and of a more negatively charged derivative were determined. We found that the C(Pd) of cytoplasmic soluble GFP and cytoplasmic forms of modified GFP were the most strongly correlated with R(S) and that the apparent aberrant increase in C(Pd) of a negatively charged GFP derivative was, at least in part, the result of the charge effect on R(S).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.