ArticleParasitology research2008
Protease activity in extracellular products secreted in vitro by trophozoites of Giardia duodenalis.
Article in Parasitology research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 41 citations in OpenAlex.
- Gut bacteria influence Blastocystis sp. phenotypes and may trigger pathogenicity.PLoS neglected tropical diseases · 2023Article
- Hemoglobin uptake and utilization by human protozoan parasites: a review.Frontiers in cellular and infection microbiology · 2023Review
- Giardia lamblia Decreases NF-κB p65Scientific reports · 2020Article
- High Cysteine Membrane Proteins (HCMPs) Are Up-Regulated DuringFrontiers in genetics · 2020Article
- Recent insights into innate and adaptive immune responses to Giardia.Advances in parasitology · 2019Review
- Secreted Giardia intestinalis cysteine proteases disrupt intestinal epithelial cell junctional complexes and degrade chemokines.Virulence · 2018Article
- Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells.PLoS neglected tropical diseases · 2017Article
- The Intersection of Immune Responses, Microbiota, and Pathogenesis in Giardiasis.Trends in parasitology · 2017Review
- Article
- Giardia duodenalis cathepsin B proteases degrade intestinal epithelial interleukin-8 and attenuate interleukin-8-induced neutrophil chemotaxis.Infection and immunity · 2014Article
- Amoebic forms of Blastocystis spp. - evidence for a pathogenic role.Parasites & vectors · 2013Article
- Host-parasite interaction: parasite-derived and -induced proteases that degrade human extracellular matrix.Journal of parasitology research · 2012Article
- An atypical proprotein convertase in Giardia lamblia differentiation.Molecular and biochemical parasitology · 2011Article
- In vitro antigiardial activity of the cysteine protease inhibitor E-64.Revista do Instituto de Medicina Tropical de Sao PauloArticle
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There are evidences that Giardia trophozoites contain and/or release proteolytic enzymes that may be implicated in pathogenesis of giardiasis. This report describes a preliminary characterization of the proteolytic activity in excretory/secretory (E/S) products of Giardia duodenalis trophozoites of an axenic Brazilian strain (BTU-11) and the reference strain Portland 1 (P1). The protease activity of E/S products in conditioned medium by trophozoites of each strain was analyzed using substrate (gelatin and collagen) impregnated SDS-PAGE and hemoglobin assay. The protease characterization was based on inhibition assays including synthetic inhibitors. Proteolytic products were detected in the conditioned medium by trophozoites of both assayed strains. In the gels containing copolymerized gelatin and collagen, E/S products promoted degradation of the substrates and the most evident proteolysis zones were distributed in the migration regions of 77 to 18 kDa and 145 to 18 kDa, respectively, in the patterns of gelatinolytic and collagenolytic activities. Degradation of hemoglobin was also observed, and the pattern of hydrolysis was similar in both E/S products assayed. Inhibition assays showed that the main proteolytic activity in both E/S products is due to cysteine proteases although the presence of serine proteases was also indicated, mainly in the hydrolysis of hemoglobin.
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