ArticlePlant cell reports2008
Expression of active Streptomyces phage phiC31 integrase in transgenic wheat plants.
Article in Plant cell reports, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 34 citations in OpenAlex.
- The evolving landscape of precise DNA insertion in plants.Nature communications · 2025Review
- Exploring a general multi-pronged activation strategy for natural product discovery in Actinomycetes.Communications biology · 2024Article
- Genetic switches designed for eukaryotic cells and controlled by serine integrases.Communications biology · 2020Article
- Site-specific recombinase genome engineering toolkit in maize.Plant direct · 2020Article
- Gene stacking by recombinases.Plant biotechnology journal · 2016Review
- Engineering of plant chromosomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- Split-gene system for hybrid wheat seed production.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Site-directed zebrafish transgenesis into single landing sites with the phiC31 integrase system.Developmental dynamics : an official publication of the American Association of Anatomists · 2013Article
- Article
- Recombinase technology: applications and possibilities.Plant cell reports · 2011Review
- Use of phage φC31 integrase as a tool for zebrafish genome manipulation.Methods in cell biology · 2011Article
- Transgene excision from wheat chromosomes by phage phiC31 integrase.Plant molecular biology · 2010Article
- PhiC31 recombination system demonstrates heritable germinal transmission of site-specific excision from the Arabidopsis genome.BMC biotechnology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Site-specific recombination systems are becoming an important tool for the genetic modification of crop plants. Here we report the functional expression of the Streptomyces phage-derived phiC31 recombinase (integrase) in wheat. T-DNA constructs containing a phiC31 integrase transgene were stably transformed into wheat plants via particle gun bombardment. A plant-virus-based assay system was used to monitor the site-specific recombination activity of the recombinant integrase protein in vivo. We established several independent doubled haploid (DH) inbred lines that constitutively express an active integrase enzyme without any apparent detrimental effects on plant growth and development. The potential of phiC31 integrase expression in crop plants related to transgene control technologies or hybrid breeding systems is discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.