Evidence map›Paper›PMID 18797838›Full record

ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2008

New DNA markers for high molecular weight glutenin subunits in wheat.

Sixin Liu, Shiaoman Chao, James A Anderson

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Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

32 citing papers in PubMed.

  1. The Role ofInternational journal of molecular sciences · 2025
    Review
  2. Article
  3. Identification of a novel QTL associated with Fusarium head blight resistance on chromosome 3B in common wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025
    Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Novel Genetic Loci fromInternational journal of molecular sciences · 2023
    Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Review
  14. Article
  15. SNP markers for early identification of high molecular weight glutenin subunits (HMW-GSs) in bread wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020
    Article
  16. Article
  17. Article
  18. Article
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4 · The record

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5 · Who and what money

Authors and funding

3 authors.

Sixin LiuDepartment of Agronomy and Plant Genetics, University of Minnesota, St Paul, MN, 55108, USA.
Shiaoman Chao
James A Anderson

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

End-use quality is one of the priorities of modern wheat (Triticum aestivum L.) breeding. Even though quality is a complex trait, high molecular weight (HMW) glutenins play a major role in determining the bread making quality of wheat. DNA markers developed from the sequences of HMW glutenin genes were reported in several previous studies to facilitate marker-assisted selection (MAS). However, most of the previously available markers are dominant and amplify large DNA fragments, and thus are not ideal for high throughput genotyping using modern equipment. The objective of this study was to develop and validate co-dominant markers suitable for high throughput MAS for HMW glutenin subunits encoded at the Glu-A1 and Glu-D1 loci. Indels were identified by sequence alignment of allelic HMW glutenin genes, and were targeted to develop locus-specific co-dominant markers. Marker UMN19 was developed by targeting an 18-bp deletion in the coding sequence of subunit Ax2* of Glu-A1. A single DNA fragment was amplified by marker UMN19, and was placed onto chromosome 1AL. Sixteen wheat cultivars with known HMW glutenin subunits were used to validate marker UMN19. The cultivars with subunit Ax2* amplified the 362-bp fragment as expected, and a 344-bp fragment was observed for cultivars with subunit Ax1 or the Ax-null allele. Two co-dominant markers, UMN25 and UMN26, were developed for Glu-D1 by targeting the fragment size polymorphic sites between subunits Dx2 and Dx5, and between Dy10 and Dy12, respectively. The 16 wheat cultivars with known HMW glutenin subunit composition were genotyped with markers UMN25 and UMN26, and the genotypes perfectly matched their subunit types. Using an Applied Biosystems 3130xl Genetic Analyzer, four F(2) populations segregating for the Glu-A1 or Glu-D1 locus were successfully genotyped with primers UMN19, UMN25 and UMN26 labeled with fluorescent dyes.

Indexed as

Genetic MarkersBase SequenceDNA, PlantGenes, PlantGenotypeGlutensMolecular Sequence DataSequence AlignmentTriticumDNA, PlantGenetic MarkersgluteninGlutens

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.