ArticleProceedings of the National Academy of Sciences of the United States of America2008
Mutations alter the sodium versus proton use of a Bacillus clausii flagellar motor and confer dual ion use on Bacillus subtilis motors.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 55 citations in OpenAlex.
- Ion-Powered Rotary Motors: Where Did They Come from and Where They Are Going?International journal of molecular sciences · 2023Review
- The rapid evolution of flagellar ion selectivity in experimental populations ofScience advances · 2022Article
- Novel transient cytoplasmic rings stabilize assembling bacterial flagellar motors.The EMBO journal · 2022Article
- The Dynamic Ion Motive Force Powering the Bacterial Flagellar Motor.Frontiers in microbiology · 2021Review
- Coupling Ion Specificity of the Flagellar Stator Proteins MotA1/MotB1 ofBiomolecules · 2020Article
- MotP Subunit is Critical for Ion Selectivity and Evolution of a KBiomolecules · 2020Article
- Analysis of the Complete Genome of the Alkaliphilic and Phototrophic FirmicuteMicroorganisms · 2020Article
- Evolution of the Stator Elements of Rotary Prokaryote Motors.Journal of bacteriology · 2020Review
- Dynamic exchange of two types of stator units inComputational and structural biotechnology journal · 2020Review
- Ancestral Sequence Reconstructions of MotB Are Proton-Motile and Require MotA for Motility.Frontiers in microbiology · 2020Article
- Flagella-Driven Motility of Bacteria.Biomolecules · 2019Review
- The presence and absence of periplasmic rings in bacterial flagellar motors correlates with stator type.eLife · 2019Article
- The Role ofFrontiers in cellular and infection microbiology · 2019Article
- Nonconventional cation-coupled flagellar motors derived from the alkaliphilic Bacillus and Paenibacillus species.Extremophiles : life under extreme conditions · 2017Review
- The Bacterial Flagellar Type III Export Gate Complex Is a Dual Fuel Engine That Can Use Both H+ and Na+ for Flagellar Protein Export.PLoS pathogens · 2016Article
- A novel type bacterial flagellar motor that can use divalent cations as a coupling ion.Scientific reports · 2016Article
- Serine 26 in the PomB subunit of the flagellar motor is essential for hypermotility of Vibrio cholerae.PloS one · 2015Article
- Structure and function of the bi-directional bacterial flagellar motor.Biomolecules · 2014Review
- Genome Sequence of the Extreme Obligate Alkaliphile Bacillus marmarensis Strain DSM 21297.Genome announcements · 2013Article
- The ferredoxin:NAD+ oxidoreductase (Rnf) from the acetogen Acetobacterium woodii requires Na+ and is reversibly coupled to the membrane potential.The Journal of biological chemistry · 2013Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Bacterial flagella contain membrane-embedded stators, Mot complexes, that harness the energy of either transmembrane proton or sodium ion gradients to power motility. Use of sodium ion gradients is associated with elevated pH and sodium concentrations. The Mot complexes studied to date contain channels that use either protons or sodium ions, with some bacteria having only one type and others having two distinct Mot types with different ion-coupling. Here, alkaliphilic Bacillus clausii KSM-K16 was shown to be motile in a pH range from 7 to 11 although its genome encodes only one Mot (BCl-MotAB). Assays of swimming as a function of pH, sodium concentration, and ion-selective motility inhibitors showed that BCl-MotAB couples motility to sodium at the high end of its pH range but uses protons at lower pH. This pattern was confirmed in swimming assays of a statorless Bacillus subtilis mutant expressing either BCl-MotAB or one of the two B. subtilis stators, sodium-coupled Bs-MotPS or proton-coupled Bs-MotAB. Pairs of mutations in BCl-MotB were identified that converted the naturally bifunctional BCl-MotAB to stators that preferentially use either protons or sodium ions across the full pH range. We then identified trios of mutations that added a capacity for dual-ion coupling on the distinct B. subtilis Bs-MotAB and Bs-MotPS motors. Determinants that alter the specificity of bifunctional and single-coupled flagellar stators add to insights from studies of other ion-translocating transporters that use both protons and sodium ions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.