ArticleMolecular and cellular biology2008
Mechanistic insights into replication termination as revealed by investigations of the Reb1-Ter3 complex of Schizosaccharomyces pombe.
Article in Molecular and cellular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- Review
- Functional architecture of the Reb1-Ter complex of Schizosaccharomyces pombe.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Crystallization and preliminary X-ray characterization of the eukaryotic replication terminator Reb1-Ter DNA complex.Acta crystallographica. Section F, Structural biology communications · 2015Article
- The extent of error-prone replication restart by homologous recombination is controlled by Exo1 and checkpoint proteins.Journal of cell science · 2014Article
- Mechanism and physiological significance of programmed replication termination.Seminars in cell & developmental biology · 2014Review
- Impediments to replication fork movement: stabilisation, reactivation and genome instability.Chromosoma · 2013Review
- Telomeres and viruses: common themes of genome maintenance.Frontiers in oncology · 2012Article
- Repeat expansion in the budding yeast ribosomal DNA can occur independently of the canonical homologous recombination machinery.Nucleic acids research · 2011Article
- The replisome pausing factor Timeless is required for episomal maintenance of latent Epstein-Barr virus.Journal of virology · 2011Article
- Article
- "Chromosome kissing" and modulation of replication termination.Bioarchitecture · 2011Article
- Chromosomes kiss and tell: Long-range chromosome contacts modulate replication termination.Cell cycle (Georgetown, Tex.) · 2010Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Relatively little is known about the interaction of eukaryotic replication terminator proteins with the cognate termini and the replication termination mechanism. Here, we report a biochemical analysis of the interaction of the Reb1 terminator protein of Schizosaccharomyces pombe, which binds to the Ter3 site present in the nontranscribed spacers of ribosomal DNA, located in chromosome III. We show that Reb1 is a dimeric protein and that the N-terminal dimerization domain of the protein is dispensable for replication termination. Unlike its mammalian counterpart Ttf1, Reb1 did not need an accessory protein to bind to Ter3. The two myb/SANT domains and an adjacent, N-terminal 154-amino-acid-long segment (called the myb-associated domain) were both necessary and sufficient for optimal DNA binding in vitro and fork arrest in vivo. The protein and its binding site Ter3 were unable to arrest forks initiated in vivo from ars of Saccharomyces cerevisiae in the cell milieu of the latter despite the facts that the protein retained the proper affinity of binding, was located in vivo at the Ter site, and apparently was not displaced by the "sweepase" Rrm3. These observations suggest that replication fork arrest is not an intrinsic property of the Reb1-Ter3 complex.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.