ArticleMolecular and cellular biology2008
Drosophila maternal Hsp83 mRNA destabilization is directed by multiple SMAUG recognition elements in the open reading frame.
Article in Molecular and cellular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Oncogenic Role of SAMD4B in Breast Cancer Progression by Activating Wnt/β-Catenin Pathway.Biomolecules · 2025Article
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- The role of structure in regulatory RNA elements.Bioscience reports · 2024Review
- Mechanistic insights into the basis of widespread RNA localization.Nature cell biology · 2024Review
- RNA binding protein SAMD4: current knowledge and future perspectives.Cell & bioscience · 2023Review
- RNA-binding protein SAMD4A inhibits breast tumor angiogenesis by modulating the balance of angiogenesis program.Cancer science · 2021Article
- Intracellular mRNA transport and localized translation.Nature reviews. Molecular cell biology · 2021Review
- Molecular asymmetry in the cephalochordate embryo revealed by single-blastomere transcriptome profiling.PLoS genetics · 2020Article
- Molecular mechanisms behind mRNA localization in axons.Open biology · 2020Review
- Mechanisms and consequences of subcellular RNA localization across diverse cell types.Traffic (Copenhagen, Denmark) · 2020Review
- Organizing the oocyte: RNA localization meets phase separation.Current topics in developmental biology · 2020Article
- Post-translational regulation of the maternal-to-zygotic transition.Cellular and molecular life sciences : CMLS · 2018Review
- Flying the RNA Nest: Drosophila Reveals Novel Insights into the Transcriptome Dynamics of Early Development.Journal of developmental biology · 2018Review
- RNA-binding protein SAMD4 regulates skeleton development through translational inhibition of Mig6 expression.Cell discovery · 2017Article
- The Smaug RNA-Binding Protein Is Essential for microRNA Synthesis During theG3 (Bethesda, Md.) · 2016Article
- The Drosophila prage Gene, Required for Maternal Transcript Destabilization in Embryos, Encodes a Predicted RNA Exonuclease.G3 (Bethesda, Md.) · 2016Article
- A Smaug2-Based Translational Repression Complex Determines the Balance between Precursor Maintenance versus Differentiation during Mammalian Neurogenesis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Germ Line Versus Soma in the Transition from Egg to Embryo.Current topics in developmental biology · 2015Review
- The Maternal-to-Zygotic Transition During Vertebrate Development: A Model for Reprogramming.Current topics in developmental biology · 2015Review
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
SMAUG (SMG) is an RNA-binding protein that functions as a key component of a transcript degradation pathway that eliminates maternal mRNAs in the bulk cytoplasm of activated Drosophila melanogaster eggs. We previously showed that SMG destabilizes maternal Hsp83 mRNA by recruiting the CCR4-NOT deadenylase to trigger decay; however, the cis-acting elements through which this was accomplished were unknown. Here we show that Hsp83 transcript degradation is regulated by a major element, the Hsp83 mRNA instability element (HIE), which maps to a 615-nucleotide region of the open reading frame (ORF). The HIE is sufficient for association of a transgenic mRNA with SMG protein as well as for SMG-dependent destabilization. Although the Hsp83 mRNA is translated in the early embryo, we show that translation of the mRNA is not necessary for destabilization; indeed, the HIE functions even when located in an mRNA's 3' untranslated region. The Hsp83 mRNA contains eight predicted SMG recognition elements (SREs); all map to the ORF, and six reside within the HIE. Mutation of a single amino acid residue that is essential for SMG's interaction with SREs stabilizes endogenous Hsp83 transcripts. Furthermore, simultaneous mutation of all eight predicted SREs also results in transcript stabilization. A plausible model is that the multiple, widely distributed SREs in the ORF enable some SMG molecules to remain bound to the mRNA despite ribosome transit through any individual SRE. Thus, SMG can recruit the CCR4-NOT deadenylase to trigger Hsp83 mRNA degradation despite the fact that it is being translated.
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