ArticleClinical cancer research : an official journal of the American Association for Cancer Research2008
Gene expression profiling reveals similarities between the in vitro and in vivo responses of immune effector cells to IFN-alpha.
Article in Clinical cancer research : an official journal of the American Association for Cancer Research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03117816 (Efficacy and Safety of Pegylated Proline Interferon Alpha 2b), which is not on this map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Efficacy and Safety of Pegylated Proline Interferon Alpha 2b (AOP2014) in Maintaining Deep Molecular Remissions in Patients With Chronic Myeloid Leukemia (CML) Who Discontinue ABL-Kinase Inhibitor Therapy - a Randomized Phase III, Multicenter Trial With Post-study Follow-up
Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- Phase II Trial of Interleukin-12 Followed by Interferon Alfa-2b in Patients with Metastatic Malignant Melanoma: Results from CALGB 500001 (Alliance).Cancer immunology research · 2026Trial
- Gene regulatory and clinical effects of interferon β in patients with metastatic melanoma: a phase II trial.Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research · 2011Trial
- Single cell and TCR analysis of immune cells from AAV gene therapy-dosed Duchenne muscular dystrophy patients.Molecular therapy. Methods & clinical development · 2024Article
- C19orf66 Inhibits Japanese Encephalitis Virus Replication by Targeting -1 PRF and the NS3 Protein.Virologica Sinica · 2021Article
- ROS Generation in Microglia: Understanding Oxidative Stress and Inflammation in Neurodegenerative Disease.Antioxidants (Basel, Switzerland) · 2020Review
- TLDc proteins: new players in the oxidative stress response and neurological disease.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Review
- Article
- Recombinant Human IFNα-2b Response Promotes Vaginal Epithelial Cells Defense againstFrontiers in microbiology · 2017Article
- Immune cell subsets and their gene expression profiles from human PBMC isolated by Vacutainer Cell Preparation Tube (CPT™) and standard density gradient.BMC immunology · 2015Article
- Widely divergent transcriptional patterns between SLE patients of different ancestral backgrounds in sorted immune cell populations.Journal of autoimmunity · 2015Article
- In vitro blood cell responsiveness to IFN-α predicts clinical response independently of IL28B in hepatitis C virus genotype 1 infected patients.Journal of translational medicine · 2014Article
- Melanoma NOS1 expression promotes dysfunctional IFN signaling.The Journal of clinical investigation · 2014Article
- Role of microRNA-15a in autoantibody production in interferon-augmented murine model of lupus.Molecular immunology · 2012Article
- Plasmacytoid dendritic cells and C1q differentially regulate inflammatory gene induction by lupus immune complexes.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Concomitant detection of IFNα signature and activated monocyte/dendritic cell precursors in the peripheral blood of IFNα-treated subjects at early times after repeated local cytokine treatments.Journal of translational medicine · 2011Article
- Microarray analysis for a comprehensive immunological-status evaluation during cancer vaccine immune monitoring.Journal of biomedicine & biotechnology · 2011Review
- Article
- Identification of alpha interferon-induced genes associated with antiviral activity in Daudi cells and characterization of IFIT3 as a novel antiviral gene.Journal of virology · 2010Article
- Dissecting interferon-induced transcriptional programs in human peripheral blood cells.PloS one · 2010Article
- Interleukin-29 binds to melanoma cells inducing Jak-STAT signal transduction and apoptosis.Molecular cancer therapeutics · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
purposeThe precise molecular targets of IFN-alpha therapy in the context of malignant melanoma are unknown but seem to involve signal transducers and activators of transcription 1 signal transduction within host immune effector cells. We hypothesized that the in vitro transcriptional response of patient peripheral blood mononuclear cells (PBMC) to IFN-alpha would be similar to the in vivo response to treatment with high-dose IFN-alpha. EXPERIMENTAL
designThe gene expression profiles of PBMCs and immune cell subsets treated in vitro with IFN-alpha were evaluated, as were PBMCs obtained from melanoma patients receiving adjuvant IFN-alpha.
resultsTwenty-seven genes were up-regulated in PBMCs from normal donors after treatment with IFN-alpha in vitro for 18 hours (>2-fold, P < 0.001). A subset of these genes (in addition to others) was significantly expressed in IFN-alpha-treated T cells, natural killer cells, and monocytes. Analysis of gene expression within PBMCs from melanoma patients (n = 13) receiving high-dose IFN-alpha-2b (20 MU/m(2) i.v.) revealed significant up-regulation (>2-fold) of 21 genes (P < 0.001). Also, the gene expression profile of in vitro IFN-alpha-stimulated patient PBMCs was similar to that of PBMCs obtained from the same patient after IFN-alpha therapy.
conclusionsThis report is the first to describe the transcriptional response of T cells, natural killer cells, and monocytes to IFN-alpha and characterize the transcriptional profiles of PBMCs from melanoma patients undergoing IFN-alpha immunotherapy. In addition, it was determined that microarray analysis of patient PBMCs after in vitro stimulation with IFN-alpha may be a useful predictor of the in vivo response of immune cells to IFN-alpha immunotherapy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.