ArticleBrain research2008
An automated segmentation methodology for quantifying immunoreactive puncta number and fluorescence intensity in tissue sections.
Article in Brain research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Hyperphosphorylated tau is elevated in Alzheimer's disease with psychosis.Journal of Alzheimer's disease : JAD · 2014Trial
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- Aging disrupts the coordination between mRNA and protein expression in mouse and human midbrain.Molecular psychiatry · 2025Article
- Aging disrupts the coordination between mRNA and protein expression in mouse and human midbrain.bioRxiv : the preprint server for biology · 2024Article
- Terminal type-specific cannabinoid CB1 receptor alterations in patients with schizophrenia: A pilot study.Neurobiology of disease · 2023Article
- Terminal type-specific cannabinoid CB1 receptor alterations in patients with schizophrenia: a pilot study.bioRxiv : the preprint server for biology · 2023Article
- Vesicular Glutamate Transporter Changes in the Cortical Default Mode Network During the Clinical and Pathological Progression of Alzheimer's Disease.Journal of Alzheimer's disease : JAD · 2023Article
- Cell type specific cannabinoid CB1 receptor distribution across the human and non-human primate cortex.Scientific reports · 2022Article
- Phenotype and Distribution of Immature Neurons in the Human Cerebral Cortex Layer II.Frontiers in neuroanatomy · 2022Article
- Basic quantitative morphological methods applied to the central nervous system.The Journal of comparative neurology · 2021Review
- Laminar Differences in the Targeting of Dendritic Spines by Cortical Pyramidal Neurons and Interneurons in Human Dorsolateral Prefrontal Cortex.Neuroscience · 2021Article
- GABA bouton subpopulations in the human dentate gyrus are differentially altered in mesial temporal lobe epilepsy.Journal of neurophysiology · 2020Article
- Automated Slide Scanning and Segmentation in Fluorescently-labeled Tissues Using a Widefield High-content Analysis System.Journal of visualized experiments : JoVE · 2018Article
- MultiMap: A Tool to Automatically Extract and Analyse Spatial Microscopic Data From Large Stacks of Confocal Microscopy Images.Frontiers in neuroanatomy · 2018Article
- Loss of precuneus dendritic spines immunopositive for spinophilin is related to cognitive impairment in early Alzheimer's disease.Neurobiology of aging · 2017Article
- Automating cell detection and classification in human brain fluorescent microscopy images using dictionary learning and sparse coding.Journal of neuroscience methods · 2017Article
- Stereological Assessments of Neuronal Pathology in Auditory Cortex in Schizophrenia.Frontiers in neuroanatomy · 2017Review
- GABA-Synthesizing Enzymes in Calbindin and Calretinin Neurons in Monkey Prefrontal Cortex.Cerebral cortex (New York, N.Y. : 1991) · 2016Article
- Developmental Trajectories of Auditory Cortex Synaptic Structures and Gap-Prepulse Inhibition of Acoustic Startle Between Early Adolescence and Young Adulthood in Mice.Cerebral cortex (New York, N.Y. : 1991) · 2016Article
- Megakaryocytic Maturation in Response to Shear Flow Is Mediated by the Activator Protein 1 (AP-1) Transcription Factor via Mitogen-activated Protein Kinase (MAPK) Mechanotransduction.The Journal of biological chemistry · 2016Article
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Authors and funding
4 authors.
Funding
Abstract
A number of human brain diseases have been associated with disturbances in the structure and function of cortical synapses. Answering fundamental questions about the synaptic machinery in these disease states requires the ability to image and quantify small synaptic structures in tissue sections and to evaluate protein levels at these major sites of function. We developed a new automated segmentation imaging method specifically to answer such fundamental questions. The method takes advantage of advances in spinning disk confocal microscopy, and combines information from multiple iterations of a fluorescence intensity/morphological segmentation protocol to construct three-dimensional object masks of immunoreactive (IR) puncta. This new methodology is unique in that high- and low-fluorescing IR puncta are equally masked, allowing for quantification of the number of fluorescently-labeled puncta in tissue sections. In addition, the shape of the final object masks highly represents their corresponding original data. Thus, the object masks can be used to extract information about the IR puncta (e.g., average fluorescence intensity of proteins of interest). Importantly, the segmentation method presented can be easily adapted for use with most existing microscopy analysis packages.
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