Evidence map›Paper›PMID 18793351›Full record

ArticleJournal of cellular and molecular medicine2009

Cardioprotection by adaptation to ischaemia augments autophagy in association with BAG-1 protein.

Narasimman Gurusamy, Istvan Lekli, Nikolai V Gorbunov, Mihaela Gherghiceanu, Lawrence M Popescu, Dipak K Das

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular and molecular medicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.

0numbers the graph read from it
0cells of the map it votes in
89citing papers in PubMed
7.6field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

89 citing papers in PubMed, 152 citations in OpenAlex.

  1. Trial
  2. Article
  3. Article
  4. Article
  5. Autophagy in Heart Failure: Insights into Mechanisms and Therapeutic Implications.Journal of cardiovascular development and disease · 2023
    Review
  6. Article
  7. Review
  8. Regulation of autophagy of the heart in ischemia and reperfusion.Apoptosis : an international journal on programmed cell death · 2023
    Review
  9. Article
  10. Review
  11. Chaperone-mediated autophagy protects cardiomyocytes against hypoxic-cell death.American journal of physiology. Cell physiology · 2022
    Article
  12. Review
  13. Article
  14. Review
  15. Article
  16. Article
  17. Review
  18. Article
  19. Review
  20. Article

29 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Narasimman GurusamyCardiovascular Research Center, University of Connecticut School of Medicine, Farmington, 06030-1110, USA.
Istvan Lekli
Nikolai V Gorbunov
Mihaela Gherghiceanu
Lawrence M Popescu
Dipak K Das
University of Connecticut · USCarol Davila University of Medicine and Pharmacy · ROInstitutul National Victor Babes · RO

Funding

PHOSPHOLIPIDS IN MYOCARDIAL REPERFUSION INJURYR01HL034360 · NHLBI · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI DAS, DIPAK K · 1987 to 2009
$2.8M
MYOCARDIAL PRESERVATION DURING ISCHEMIC ARRESTR01HL022559 · NHLBI · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI DAS, DIPAK K · 1985 to 2008
$2.7M
SUPEROXIDE RADICALS IN REPERFUSION INJURYR01HL033889 · NHLBI · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI DAS, DIPAK K · 1986 to 1988
–
NHLBI NIH HHS HL22559NHLBI NIH HHS HL33889NHLBI NIH HHS HL 34360NHLBI NIH HHS R01 HL022559NHLBI NIH HHS R01 HL034360
6 · The paper itself

Abstract

Autophagy is an intracellular process in which a cell digests its own constituents via lysosomal degradative pathway. Though autophagy has been shown in several cardiac diseases like heart failure, hypertrophy and ischaemic cardiomyopathy, the role and the regulation of autophagy is still largely unknown. Bcl-2-associated athanogene (BAG-1) is a multifunctional pro-survival molecule that binds with Hsp70/Hsc70. In this study, myocardial adaptation to ischaemia by repeated brief episodes of ischaemia and reperfusion (I/R) prior to lethal I/R enhanced the expression of autophagosomal membrane specific protein light chain 3 (LC3)-II, and Beclin-1, a molecule involved in autophagy and BAG-1. Autophagosomes structures were found in the adapted myocardium through electron microscopy. Co-immunoprecipitation and co-immunofluorescence analyses revealed that LC3-II was bound with BAG-1. Inhibition of autophagy by treating rats with Wortmannin (15 microg/kg; intraperitoneally) abolished the ischaemic adaptation-induced induction of LC3-II, Beclin-1, BAG-1 and cardioprotection. Intramyocardial injection of BAG-1 siRNA attenuated the induction of LC3-II, and abolished the cardioprotection achieved by adaptation. Furthermore, hypoxic adaptation in cardiac myoblast cells induced LC3-II and BAG-1. BAG-1 siRNA treatment attenuated hypoxic adaptation-induced LC3-II and BAG-1, and abolished improvement in cardiac cell survival and reduction of cell death. These results clearly indicate that myocardial protection elicited by adaptation is mediated at least in part via up-regulation of autophagy in association with BAG-1 protein.

Indexed as

AndrostadienesAnimalsApoptosis Regulatory ProteinsAutophagyBeclin-1Cardiotonic AgentsCells, CulturedDNA-Binding ProteinsIschemiaIschemic Preconditioning, MyocardialMaleMicrotubule-Associated ProteinsMyocardiumRatsRats, Sprague-DawleyRNA, Small InterferingAndrostadienesAnxa3 protein, ratApoptosis Regulatory ProteinsBCL2-associated athanogene 1 proteinBeclin-1Becn1 protein, ratCardiotonic AgentsDNA-Binding ProteinsMicrotubule-Associated ProteinsRNA, Small InterferingTranscription FactorsWortmannin

Identifiers

PMID18793351
PMCPMC3823363
OpenAlexW2040482760

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.