ArticleJournal of neurochemistry2008
Nerve growth factor regulates the expression of the cholinergic locus and the high-affinity choline transporter via the Akt/PKB signaling pathway.
Article in Journal of neurochemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- Network pharmacology, molecular docking andIBRO neuroscience reports · 2026Article
- Choline metabolism drives metastasis in BRCA1-deficient ovarian cancers by activating FAM3C.Nature communications · 2026Article
- Reduction of acetylcholine in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice.Scientific reports · 2021Article
- Nerve Growth Factor in Alcohol Use Disorders.Current neuropharmacology · 2021Review
- Growth factors-based therapeutic strategies and their underlying signaling mechanisms for peripheral nerve regeneration.Acta pharmacologica Sinica · 2020Review
- The Regulatory Effects of Acetyl-CoA Distribution in the Healthy and Diseased Brain.Frontiers in cellular neuroscience · 2018Review
- Neurotrophin Signaling and Stem Cells-Implications for Neurodegenerative Diseases and Stem Cell Therapy.Molecular neurobiology · 2017Review
- Fermented Citrus reticulata (ponkan) fruit squeezed draff that contains a large amount of 4'-demethylnobiletin prevents MK801-induced memory impairment.Journal of natural medicines · 2017Article
- Protective Role of Quercetin in Cadmium-Induced Cholinergic Dysfunctions in Rat Brain by Modulating Mitochondrial Integrity and MAP Kinase Signaling.Molecular neurobiology · 2017Article
- Prenatal Exposure of Cypermethrin Induces Similar Alterations in Xenobiotic-Metabolizing Cytochrome P450s and Rate-Limiting Enzymes of Neurotransmitter Synthesis in Brain Regions of Rat Offsprings During Postnatal Development.Molecular neurobiology · 2016Article
- Crosstalk among electrical activity, trophic factors and morphogenetic proteins in the regulation of neurotransmitter phenotype specification.Journal of chemical neuroanatomy · 2016Review
- Article
- Diminished trkA receptor signaling reveals cholinergic-attentional vulnerability of aging.The European journal of neuroscience · 2013Article
- Substrate-induced internalization of the high-affinity choline transporter.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- p38α and p38β mitogen-activated protein kinases determine cholinergic transdifferentiation of sympathetic neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Nuclear choline acetyltransferase activates transcription of a high-affinity choline transporter.The Journal of biological chemistry · 2011Article
- Β-amyloid 1-42 oligomers impair function of human embryonic stem cell-derived forebrain cholinergic neurons.PloS one · 2010Article
- Analysis of Gene Expression and Neuronal Phenotype in Neuroscreen-1 (NS-1) Cells.International journal of biomedical investigationArticle
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Nerve growth factor (NGF) is a trophic and survival factor for cholinergic neurons, and it induces the expression of several genes that are essential for synthesis and storage of acetylcholine (ACh), specifically choline acetyltransferase, vesicular ACh transporter (VAChT), and choline transporter. We have found previously that the phosphatidylinositol 3'-kinase pathway, but not the MEK/MAPK pathway, is the mediator of NGF-induced cholinergic differentiation. Here we demonstrate, in the rat pheochromocytoma cell line PC12 and in primary mouse neuronal cultures, that NGF-evoked up-regulation of these three cholinergic-specific genes is mediated by the anti-apoptotic signaling molecule Akt/protein kinase B. Inhibition of Akt activation by the pharmacological inhibitor 1L-6-hydroxymethyl-chiro-inositol 2(R)-2-O-methyl-3-O-octadecylcarbonate (HIMO), or by a peptide fragment derived from the proto-oncogene TLC1, eliminated NGF-stimulated increases in cholinergic gene expression, as demonstrated by RT-PCR and reporter gene assays. Moreover, treatment with HIMO reversed NGF-evoked increases in choline acetyltransferase activity and ACh production. In co-transfection assays with the reporter construct, a dominant-negative Akt plasmid and Akt1-specific small interfering RNA also attenuated NGF-induced cholinergic promoter activity. Our data indicate that, in addition to its well-described role in promoting neuronal survival, Akt can also mediate signals necessary for neurochemical differentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.