ArticleMolecular biology reports2009
Improvement of efficiency of genetic transformation for Dunaliella salina by glass beads method.
Article in Molecular biology reports, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 120 citations in OpenAlex.
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- The complete mitochondrial genome of the green microalgaeMitochondrial DNA. Part B, Resources · 2017Article
- The complete chloroplast genome of the green microalgaeMitochondrial DNA. Part B, Resources · 2017Article
- Efficient and stable transformation ofBioImpacts : BI · 2017Article
- The Potential for Microalgae as Bioreactors to Produce Pharmaceuticals.International journal of molecular sciences · 2016Review
- Synergistic Action of D-Glucose and Acetosyringone on Agrobacterium Strains for Efficient Dunaliella Transformation.PloS one · 2016Article
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- Production of carotenoids by microalgae: achievements and challenges.Photosynthesis research · 2015Review
- The potential of transgenic green microalgae; a robust photobioreactor to produce recombinant therapeutic proteins.World journal of microbiology & biotechnology · 2014Review
- Tipping points in seaweed genetic engineering: scaling up opportunities in the next decade.Marine drugs · 2014Review
- Protein N-glycosylation in eukaryotic microalgae and its impact on the production of nuclear expressed biopharmaceuticals.Frontiers in plant science · 2014Review
- Promoter trapping in microalgae using the antibiotic paromomycin as selective agent.Marine drugs · 2012Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dunaliella salina has been exploited as a new type of bioreactor due to its unique advantages. However, this bioreactor application was restricted for absence of a high-efficiency and stable transformation method at present. In the present study, the cells of D. salina were transformed by glass beads. The results of histochemical staining revealed that the GUS gene was successfully expressed in the positive transformants, and PCR and PCR-Southern blot analysis further demonstrated that the bar gene was integrated into the D. salina genome. Moreover, the three transformation methods, including glass beads, bombardment particle and electroporation, were compared for screening a high-efficiency transformation method for gene engineering of D. salina. The results showed that transformation efficiency of the glass beads was the highest, approximately 10(2) transformants/microg DNA. It is concluded that the established glass beads method has been demonstrated to be an optimal transformation way for D. salina.
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