ArticleApplied and environmental microbiology2008
inlA premature stop codons are common among Listeria monocytogenes isolates from foods and yield virulence-attenuated strains that confer protection against fully virulent strains.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
75 citing papers in PubMed, 152 citations in OpenAlex.
- Large-scale phenotyping and comparative genomics reveal genetic features of Listeria persistence in epithelial cells.PLoS pathogens · 2026Article
- Premature stop codons inBioscience of microbiota, food and health · 2026Article
- Bioprotective Strategies to ControlInternational journal of molecular sciences · 2025Review
- Modeling the Anti-Adhesive Role of Punicalagin Against Listeria Monocytogenes from the Analysis of the Interaction Between Internalin A and E-Cadherin.International journal of molecular sciences · 2025Article
- Exploratory Genomic Marker Analysis of Virulence Patterns inFoods (Basel, Switzerland) · 2025Article
- Molecular Characterization of Virulence-Related Genes in Listeria monocytogenes Isolated from Retail Meats in Sapporo, Japan.Current microbiology · 2025Article
- Draft genome sequences ofMicrobiology resource announcements · 2024Article
- Genomic characterization ofFrontiers in microbiology · 2024Article
- Hypervirulent clonal complex (CC) ofFrontiers in microbiology · 2024Article
- Contrasting genes conferring short- and long-term biofilm adaptation inMicrobial genomics · 2023Article
- The Slaughterhouse as Hotspot of CC1 and CC6Microorganisms · 2023Article
- Genomic and pathogenicity islands ofFrontiers in molecular biosciences · 2023Review
- Whole-Genome Sequencing-Based Characterization ofApplied and environmental microbiology · 2022Article
- Newly Designed Primers for the Sequencing of theInternational journal of molecular sciences · 2022Article
- Whole-Genome Sequence Comparisons ofMicroorganisms · 2022Article
- Review
- Whole-Genome Sequencing Reveals Multiple Subpopulations of Dominant and Persistent Lineage I Isolates ofMicroorganisms · 2022Article
- Characterization of Listeria monocytogenes isolated from wildlife in central New York.Veterinary medicine and science · 2022Article
- Prevalence and Clonal Diversity of over 1,200 Listeria monocytogenes Isolates Collected from Public Access Waters near Produce Production Areas on the Central California Coast during 2011 to 2016.Applied and environmental microbiology · 2022Article
- Article
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous studies showed that a considerable proportion of Listeria monocytogenes isolates obtained from foods carry a premature stop codon (PMSC) mutation in inlA that leads to production of a truncated and secreted InlA. To further elucidate the role these mutations play in virulence of L. monocytogenes, we created isogenic mutants, including (i) natural isolates where an inlA PMSC was reverted to a wild-type inlA allele (without a PMSC) and (ii) natural isolates where a PMSC mutation was introduced into a wild-type inlA allele; isogenic mutant sets were constructed to represent two distinct inlA PMSC mutations. Phenotypical and transcriptional analysis data showed that inlA PMSC mutations do not have a polar effect on the downstream inlB. Isogenic and natural strains carrying an inlA PMSC showed significantly reduced invasion efficiencies in Caco-2 and HepG2 cell lines as well as reduced virulence in oral guinea pig infections. Guinea pigs were also orally infected with a natural strain carrying the most common inlA PMSC mutation (vaccinated group), followed by challenge with a fully virulent L. monocytogenes strain 15 days postvaccination to probe potentially immunizing effects of exposure to L. monocytogenes with inlA PMSC mutations. Vaccinated guinea pigs showed reduced bacterial loads in internal organs and improved weight gain postchallenge, indicating reduced severity of infections in guinea pigs exposed to natural strains with inlA PMSC mutations. Our data support that (i) inlA PMSC mutations are causally associated with attenuated virulence in mammalian hosts and (ii) naturally occurring virulence-attenuated L. monocytogenes strains commonly found in food confer protective immunity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.