ArticleApplied and environmental microbiology2008
Codon optimization increases steady-state mRNA levels in Aspergillus oryzae heterologous gene expression.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 94 citations in OpenAlex.
- Heterologous protein production in filamentous fungi.Applied microbiology and biotechnology · 2023Review
- A genetic tool to express long fungal biosynthetic genes.Fungal biology and biotechnology · 2023Article
- Expression of Codon-Optimized Gene Encoding Murine Moloney Leukemia Virus Reverse Transcriptase in Escherichia coli.The protein journal · 2022Article
- Molecular characterization and secreted production of basidiomycetous cell-bound β-glycosidases applicable to production of galactooligosaccharides.Journal of industrial microbiology & biotechnology · 2022Article
- Engineered FungusJournal of fungi (Basel, Switzerland) · 2022Article
- EngineeringJournal of fungi (Basel, Switzerland) · 2021Article
- Advances in Genetic Engineering Technology and Its Application in the Industrial FungusFrontiers in microbiology · 2021Review
- Characterization of the effects of terminators and introns on recombinant gene expression in the basidiomycete Ceriporiopsis subvermispora.Journal of microbiology (Seoul, Korea) · 2020Article
- Regulating Strategies for Producing Carbohydrate Active Enzymes by Filamentous Fungal Cell Factories.Frontiers in bioengineering and biotechnology · 2020Review
- Construction of a Codon-Adapted Nourseotricin-Resistance Marker Gene for Efficient Targeted Gene Deletion in the Mycophenolic Acid ProducerJournal of fungi (Basel, Switzerland) · 2019Article
- Molecular cloning, expression and characterization of poxa1b gene from Pleurotus ostreatus.Molecular biology reports · 2019Article
- Accessibility of the Shine-Dalgarno Sequence Dictates N-Terminal Codon Bias in E. coli.Molecular cell · 2018Article
- Article
- Heterologous Biosynthesis of the Fungal Sesquiterpene Trichodermol inFrontiers in microbiology · 2018Article
- Anti-infective immunoadhesins from plants.Plant biotechnology journal · 2015Review
- The codon-optimized Δ(6)-desaturase gene of Pythium sp. as an empowering tool for engineering n3/n6 polyunsaturated fatty acid biosynthesis.BMC biotechnology · 2015Article
- Combined use of GAP and AOX1 promoters and optimization of culture conditions to enhance expression of Rhizomucor miehei lipase.Journal of industrial microbiology & biotechnology · 2015Article
- Optimized human factor IX expression cassettes for hepatic-directed gene therapy of hemophilia B.Frontiers of medicine · 2015Article
- Cellular immunity survey against urinary tract infection using pVAX/fimH cassette with mammalian and wild type codon usage as a DNA vaccine.Clinical and experimental vaccine research · 2014Article
- Selection on codon bias in yeast: a transcriptional hypothesis.Nucleic acids research · 2013Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the effect of codon optimization on the expression levels of heterologous proteins in Aspergillus oryzae, using the mite allergen Der f 7 as a model protein. A codon-optimized Der f 7 gene was synthesized according to the frequency of codon usage in A. oryzae by recursive PCR. Both native and optimized Der f 7 genes were expressed under the control of a high-level-expression promoter with their own signal peptides or in a fusion construct with A. oryzae glucoamylase (GlaA). Codon optimization markedly increased protein and mRNA production levels in both nonfused and GlaA-fused Der f 7 constructs. For constructs with native codons, analysis by 3' rapid amplification of cDNA ends revealed that poly(A) tracts tended to be added within the coding region, producing aberrant mRNAs that lack a termination codon. Insertion of a termination codon between the carrier GlaA and native Der f 7 proteins in the GlaA fusion construct resulted in increases in mRNA and secreted-carrier-GlaA levels. These results suggested that mRNAs without a termination codon as a result of premature polyadenylation are degraded, possibly through the nonstop mRNA decay pathway. We suggest that codon optimization in A. oryzae results in elimination of cryptic polyadenylation signals in native Der f 7, thereby circumventing the production of truncated transcripts and resulting in an increase in steady-state mRNA levels.
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