ArticleApplied and environmental microbiology2008
Mechanistic dissection of the enzyme complexes involved in biosynthesis of lacticin 3147 and nisin.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- Proteases Involved in Leader Peptide Removal during RiPP Biosynthesis.ACS bio & med chem Au · 2024Review
- Biosynthesis of lanthionine-constrained agonists of G protein-coupled receptors.Biochemical Society transactions · 2020Review
- Impact of the nisin modification machinery on the transport kinetics of NisT.Scientific reports · 2020Article
- Probiotics at War Against Viruses: What Is Missing From the Picture?Frontiers in microbiology · 2020Review
- A mecC allotype, mecC3, in the CoNS Staphylococcus caeli, encoded within a variant SCCmecC.The Journal of antimicrobial chemotherapy · 2019Article
- Feasability of Introducing a Thioether Ring in Vasopressin byFrontiers in microbiology · 2019Article
- Heterologous Biosynthesis, Modifications and Structural Characterization of Ruminococcin-A, a Lanthipeptide From the Gut BacteriumFrontiers in microbiology · 2018Article
- Mechanistic Understanding of Lanthipeptide Biosynthetic Enzymes.Chemical reviews · 2017Review
- Stoichiometry and structure of a lantibiotic maturation complex.Scientific reports · 2017Article
- Structure and tRNA Specificity of MibB, a Lantibiotic Dehydratase from Actinobacteria Involved in NAI-107 Biosynthesis.Cell chemical biology · 2016Article
- Synergistic binding of the leader and core peptides by the lantibiotic synthetase HalM2.ACS chemical biology · 2015Article
- Identification of essential amino acid residues in the nisin dehydratase NisB.Frontiers in microbiology · 2015Article
- In vitro activity of the nisin dehydratase NisB.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Article
- Identification of distinct nisin leader peptide regions that determine interactions with the modification enzymes NisB and NisC.FEBS open bio · 2013Article
- Substrate recognition and specificity of the NisB protein, the lantibiotic dehydratase involved in nisin biosynthesis.The Journal of biological chemistry · 2011Article
- Production of a class II two-component lantibiotic of Streptococcus pneumoniae using the class I nisin synthetic machinery and leader sequence.Antimicrobial agents and chemotherapy · 2010Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The thioether rings in the lantibiotics lacticin 3147 and nisin are posttranslationally introduced by dehydration of serines and threonines, followed by coupling of these dehydrated residues to cysteines. The prepeptides of the two-component lantibiotic lacticin 3147, LtnA1 and LtnA2, are dehydrated and cyclized by two corresponding bifunctional enzymes, LtnM1 and LtnM2, and are subsequently processed and exported via one bifunctional enzyme, LtnT. In the nisin synthetase complex, the enzymes NisB, NisC, NisT, and NisP dehydrate, cyclize, export, and process prenisin, respectively. Here, we demonstrate that the combination of LtnM2 and LtnT can modify, process, and transport peptides entirely different from LtnA2 and that LtnT can process and transport unmodified LtnA2 and unrelated peptides. Furthermore, we demonstrate a higher extent of NisB-mediated dehydration in the absence of thioether rings. Thioether rings apparently inhibited dehydration, which implies alternating actions of NisB and NisC. Furthermore, certain (but not all) NisC-cyclized peptides were exported with higher efficiency as a result of their conformation. Taken together, these data provide further insight into the applicability of Lactococcus lactis strains containing lantibiotic enzymes for the design and production of modified peptides.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.